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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Khodaei, Mohammad Mehdi Shahabadi, Nahid Kashanian, Soheila Kheirdoosh, Fahimeh |
| Description | Author Affiliation: Shahabadi N ( Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Islamic Republic of Iran. Electronic address: nahidshahabadi@yahoo.com.); Khodaei MM ( Department of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Faculty of Chemistry, Razi University, Kermanshah, Islamic Republic of Iran. Electronic address: mm_khodaei@yahoo.com.); Kashanian S ( Department of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Faculty of Chemistry, Razi University, Kermanshah, Islamic Republic of Iran.); Kheirdoosh F ( Department of Applied Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Islamic Republic of Iran.) |
| Abstract | A copper (II) complex containing aspartame (APM) as ligand, Cu(APM)2Cl2â 2H2O, was synthesized and characterized. In vitro binding interaction of this complex with native calf thymus DNA (CT-DNA) was studied at physiological pH. The interaction was studied using different methods: spectrophotometric, spectrofluorometric, competition experiment, circular dichroism (CD) and viscosimetric techniques. Hyperchromicity was observed in UV absorption band of Cu(APM)2Cl2â 2H2O. A strong fluorescence quenching reaction of DNA to Cu(APM)2Cl2â 2H2O was observed and the binding constants (Kf) and corresponding numbers of binding sites (n) were calculated at different temperatures. Thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS) were calculated to be+89.3 kJ mol(-1) and+379.3 J mol(-1) K(-1) according to Van't Hoff equation which indicated that reaction is predominantly entropically driven. Experimental results from spectroscopic methods were comparable and further supported by viscosity measurements. We suggest that Cu(APM)2Cl2â 2H2O interacts with calf thymus DNA via a groove interaction mode with an intrinsic binding constant of 8×10+4 M(-1). Binding of this copper complex to DNA was found to be stronger compared to aspartame which was studied recently. |
| ISSN | 13861425 |
| Volume Number | 120 |
| e-ISSN | 18733557 |
| Journal | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-01-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aspartame Metabolism Coordination Complexes Copper Dna Sweetening Agents Animals Analogs & Derivatives Binding Sites Cattle Circular Dichroism Chemistry Models, Molecular Nucleic Acid Conformation Spectrometry, Fluorescence Thermodynamics Journal Article Research Support, Non-u.s. Gov't Discipline Spectroscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Atomic and Molecular Physics, and Optics Analytical Chemistry Instrumentation |
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