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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Andelkovic, Boban Tesevic, Vele Raskovic, Brankica Popovic, Milica Polovic, Natalija Ostojic, Sanja |
| Description | Author Affiliation: Raskovic B ( Department of Biochemistry, University of Belgrade - Faculty of Chemistry, Studentski trg 12 - 16, 11000 Belgrade, Serbia.); Popovic M ( Department of Biochemistry, University of Belgrade - Faculty of Chemistry, Studentski trg 12 - 16, 11000 Belgrade, Serbia.); Ostojic S ( Institute of General and Physical Chemistry, Studentski trg 12, 11000 Belgrade, Serbia.); Andelkovic B ( Department of Organic Chemistry, University of Belgrade - Faculty of Chemistry, Studentski trg 12 - 16, 11000 Belgrade, Serbia.); Tesevic V ( Department of Organic Chemistry, University of Belgrade - Faculty of Chemistry, Studentski trg 12 - 16, 11000 Belgrade, Serbia.); Polovic N ( Department of Biochemistry, University of Belgrade - Faculty of Chemistry, Studentski trg 12 - 16, 11000 Belgrade, Serbia. Electronic address: polovicn@chem.bg.ac.rs.) |
| Abstract | Papain is a cysteine protease with wide substrate specificity and many applications. Despite its widespread applications, cold stability of papain has never been studied. Here, we used differential spectroscopy to monitor thermal denaturation process. Papain was the most stabile from 45 °C to 60 °C with ΔG°321 of 13.9±0.3 kJ/mol and Tm value of 84±1 °C. After cold storage, papain lost parts of its native secondary structures elements which gave an increase of 40% of intermolecular ß-sheet content (band maximum detected at frequency of 1621 cm(-1) in Fourier transform infrared (FT-IR) spectrum) indicating the presence of secondary structures necessary for aggregation. The presence of protein aggregates after cold storage was also proven by analytical size exclusion chromatography. After six freeze-thaw cycles around 75% of starting enzyme activity of papain was lost due to cold denaturation and aggregation of unfolded protein. Autoproteolysis of papain did not cause significant loss of the protein activity. Upon the cold storage, papain underwent structural rearrangements and aggregation that correspond to other cold denatured proteins, rather than autoproteolysis which could have the commercial importance for the growing polypeptide based industry. |
| ISSN | 13861425 |
| Volume Number | 150 |
| e-ISSN | 18733557 |
| Journal | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-01-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cold Temperature Papain Chemistry Preservation, Biological Protein Aggregates Protein Denaturation Amino Acid Sequence Drug Stability Enzyme Stability Molecular Sequence Data Isolation & Purification Metabolism Methods Protein Structure, Secondary Spectroscopy, Fourier Transform Infrared Journal Article Research Support, Non-u.s. Gov't Discipline Spectroscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Atomic and Molecular Physics, and Optics Analytical Chemistry Instrumentation |
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