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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sheet, Sanjoy Kumar Jamatia, Ramen Khatua, Snehadrinarayan Pal, Amarta Kumar Aguan, Kripamoy Sen, Bhaskar Thounaojam, Romita |
| Description | Country affiliation: India Author Affiliation: Sen B ( Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India.); Sheet SK ( Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India.); Thounaojam R ( Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, Meghalaya 793022, India.); Jamatia R ( Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India.); Pal AK ( Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India.); Aguan K ( Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, Meghalaya 793022, India.); Khatua S ( Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India. Electronic address: snehadri@gmail.com.) |
| Abstract | A new coumarin based Schiff base compound, CSB-1 has been synthesized to detect metal ion based on the chelation enhanced fluorescence (CHEF). The cation binding properties of CSB-1 was thoroughly examined in UV-vis and fluorescence spectroscopy. In fluorescence spectroscopy the compound showed high selectivity toward Al ion and the Al can be quantified in mixed aqueous buffer solution (MeOH: 0.01M HEPES Buffer; 9:1; v/v) at pH7.4 as well as in BSA media. The fluorescence intensity of CSB-1 was enhanced by ~24 fold after addition of only five equivalents of Al . The fluorescence titration of CSB-1 with Al in mixed aqueous buffer afforded a binding constant, K =(1.06±0.2)×10 M . The colour change from light yellow to colourless and the appearance of blue fluorescence, which can be observed by the naked eye, provides a real-time method for Al sensing. Further the live cell imaging study indicated that the detection of intracellular Al ions are also readily possible in living cell. |
| ISSN | 13861425 |
| Journal | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
| Volume Number | 173 |
| e-ISSN | 18733557 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-02-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Spectroscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Atomic and Molecular Physics, and Optics Analytical Chemistry Instrumentation |
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