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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Palumbo, Amanda J. Koivunen, Marja Tjeerdema, Ronald S. |
| Spatial Coverage | California |
| Description | Country affiliation: United States Author Affiliation: Palumbo AJ ( Department of Environmental Toxicology, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States. ajpalumbo@ucdavis.edu) |
| Abstract | Vitellogenin, the estrogen-inducible yolk protein precursor, serves as an indicator of exposure to estrogen mimicking environmental contaminants. An ELISA for the measurement of California halibut plasma vitellogenin was optimized and validated using a commercially-available antibody developed for another flatfish species, turbot. Attempts to enhance assay performance by addition of a biotinylated antibody, polyethylene glycol, and Tween-20, and altering the preincubation step are described. Inclusion of overnight preincubation was critical for low detection limits. Increasing the amount of Tween-20 to 0.05% in buffers was most effective in achieving accurate quantification of spiked plasma samples. At the IC50, the average recovery of spiked plasma samples was 104% and the interplate CV was 12%. The working range of the assay was 33-1000 ng/mL, while the detection limit in a plasma sample is 2.2 microg/mL. The performance of this assay compared very well to a homologous assay demonstrating that commercially-available antibodies can facilitate the development of bioassays for local environmentally-relevant species. The dose response relationship of halibut Vg to the model compounds 17beta-estradiol and pnonylphenol show that it is a suitable model for further studies of estrogen mimicking contaminants. |
| ISSN | 00489697 |
| Issue Number | 2 |
| Volume Number | 407 |
| e-ISSN | 18791026 |
| Journal | Science of The Total Environment |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-01-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biological Assay Methods Environmental Monitoring Enzyme-linked Immunosorbent Assay Estrogens Blood Flounder Water Pollutants, Chemical Animals Antibodies, Monoclonal Immunology Biotin Chemistry California Dose-response Relationship, Drug Estradiol Toxicity Female Phenols Polyethylene Glycols Polysorbates Time Factors Vitellogenins Journal Article Research Support, Non-u.s. Gov't Validation Studies Discipline Environmental Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Environmental Chemistry Waste Management and Disposal Pollution Environmental Engineering |
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