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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhou, Qiujun Shi, Haiyan Wang, Minghua Liu, Fengquan Li, Wei Hua, Xiude Lu, Yiwen Xia, Yazhong Liu, Xiaofeng Yin, Wei |
| Description | Author Affiliation: Hua X ( College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China); Liu X ( College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China); Yin W ( College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China); Xia Y ( Wuxi Termite Control Center, Wuxi 214071, China.); Zhou Q ( Wuxi Termite Control Center, Wuxi 214071, China.); Lu Y ( Wuxi Termite Control Center, Wuxi 214071, China.); Li W ( Wuxi Termite Control Center, Wuxi 214071, China.); Shi H ( College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China); Liu F ( College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China); Wang M ( College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China) |
| Abstract | A sensitive monoclonal antibody-based enzyme monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) was developed to detect bifenthrin in a chemical soil barrier for termite control. LBc ((2-methyl[1,1-biphenyl]-3-methoxy) carbonyl propionic acid) was conjugated to bovine serum albumin (BSA) for producing monoclonal antibody. After optimization, the IC50 and limit of detection (LOD, IC10) were 0.05 mg L(-1) and 0.004 mg L(-1), respectively. The sensitivity was improved 40-fold compared to polyclonal antibody-based ELISA reported earlier. No cross-reactivity was measured for the other analogues such as cyhalothrin, cyhalothric acid, cypermethrin, deltamethrin, fenpropathrin, fenvalerate, ethofenprox and tetramethrin except 2-methyl-3-biphenylmethyanol. Spiked recoveries were between 83.5% and 104.7% for the detection of bifenthrin in loess, red soil and black soil. All the relative standard deviations (RSDs) were less than or equal to 15.0%. Moreover, the ELISA for authentic samples showed reliability and high correlation with gas chromatography. The developed ELISA is an alternative tool for simple, sensitive and accurate monitoring of bifenthrin in chemical soil barriers. |
| ISSN | 00489697 |
| Volume Number | 502 |
| e-ISSN | 18791026 |
| Journal | Science of The Total Environment |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-01-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Environmental Monitoring Methods Enzyme-linked Immunosorbent Assay Insecticides Analysis Pyrethrins Soil Pollutants Soil Chemistry Limit Of Detection Reproducibility Of Results Journal Article Research Support, Non-u.s. Gov't Discipline Environmental Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Environmental Chemistry Waste Management and Disposal Pollution Environmental Engineering |
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