| Author |
Fernandez-Vadillo, C. Galvan, A. L. Pelaz, C. Izquierdo, F. Gomes, T. S. Pozuelo, M. J. Magnet, A. Fenoy, S. Peralta, R. H. S. Del Águila, C. |
| Description |
Country affiliation: Spain Author Affiliation: Magnet A ( Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Boadilla del Monte, Madrid, Spain); Peralta RH ( Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Boadilla del Monte, Madrid, Spain); Gomes TS ( Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Boadilla del Monte, Madrid, Spain); Izquierdo F ( Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Boadilla del Monte, Madrid, Spain); Fernandez-Vadillo C ( Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Boadilla del Monte, Madrid, Spain.); Galvan AL ( Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Boadilla del Monte, Madrid, Spain); Pozuelo MJ ( Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Boadilla del Monte, Madrid, Spain.); Pelaz C ( Laboratorio de Legionella, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.); Fenoy S ( Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Boadilla del Monte, Madrid, Spain); Del Águila C ( Facultad de Farmacia, Universidad San Pablo CEU, Urbanización Montepríncipe, Boadilla del Monte, Madrid, Spain) |
| Abstract |
Legionella spp. is the causative agent of Legionnaires' disease and is transmitted through aerosols emanating from man-made water systems. Legionella resistance to water treatments has been related to its association with environmental amoebae such as Acanthamoeba. Due to the high presence of this protozoon in Spain and the high rate of notification of Legionnaires' disease of this country, the aims of this work were to study the coexistence of these bacteria and protozoa in water as well as their interaction. The usefulness of Acanthamoeba co-culture for the isolation of environmental Legionella was also studied. For this purpose, 70 water samples were collected in 2011 from three Drinking Water Treatment Plants, three Wastewater Treatment Plants and five Natural Pools in Spain. Acanthamoeba was found by PCR in 87.1% (61/70) samples and, by culture in 85.7% (60/70) samples. Legionella was detected by PCR in 58.6% (41/70) of water samples, in 5.7% (4/70) by agar culture and 75.7% (53/70) by Acanthamoeba co-culture. From the 54 Acanthamoeba water isolates, Legionella was detected in 43 of them independently of Acanthamoeba's genotype (T3, T4 and T11). Legionella feeleii, Legionella birminghamiensis, Legionella gresilensis/berliardensis, Legionella fairfieldensis, Legionella drozanski and Legionella falloni were identified. In conclusion, our results showed that environmental Acanthamoeba is infected by Legionella to a high percentage, and due to its ubiquity, high resistance and its pathogenic potential per se, new methods for its elimination should be studied. Also, the high effectivity of Acanthamoeba co-culture for Legionella detection has been shown. |
