NDLI: Characterization and crystallization of a minimal catalytic core domain from mammalian type II adenylyl cyclase.

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Characterization and crystallization of a minimal catalytic core domain from mammalian type II adenylyl cyclase.

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Characterization and crystallization of a minimal catalytic core domain from mammalian type II adenylyl cyclase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hurley, J. H. Qin, J. Vo, B. Tang, W. J. Liu, Y. Zhang, G. Ruoho, A. E.
Description	Country affiliation: United States Author Affiliation: Zhang G ( Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0580, USA.)
Abstract	Adenylyl cyclases play a pivotal role in signal transduction by carrying out the regulated synthesis of cyclic AMP. The nine cloned mammalian adenylyl cyclases all share two conserved regions of sequence, C1 and C2, which are homologous to each other and are together responsible for catalytic activity. Recombinant C1 and C2 domains catalyze the synthesis of cyclic AMP when they are mixed and activated by forskolin, and C2 domains alone also manifest reduced levels of forskolin-stimulated enzyme activity. Using limited proteolysis and mass spectrometry, we have mapped the boundaries of a minimal stable and active C2 catalytic domain to residues 871-1090 of type II adenylyl cyclase. We report the properties and crystallization of this trimmed domain, termed IIC2-delta 4. Crystals belong to space group P4n2(1)2, where n = 1 or 3; a = b = 81.3, and c = 180.5 A; and there are two molecules per asymmetric unit related by an approximate body centering operation. Flash-frozen crystals diffract anisotropically to 2.2 A along the c* direction and to 2.8 A along the a* and b* directions using synchrotron radiation.
ISSN	09618368
e-ISSN	1469896X
Journal	Protein Science
Issue Number	4
Volume Number	6
Language	English
Publisher	Wiley-Blackwell (on behalf of The Protein Society)
Publisher Date	1997-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Adenylate Cyclase Chemistry Amino Acid Sequence Animals Catalysis Crystallization Molecular Sequence Data Protein Conformation X-ray Diffraction Research Support, U.s. Gov't, P.h.s. Discipline Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Biochemistry

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