NDLI: Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k.

Please wait, while we are loading the content...

Year: 2017 Volume: 26

Year: 2016 Volume: 25

Year: 2015 Volume: 24

Year: 2014 Volume: 23

Year: 2013 Volume: 22

Year: 2012 Volume: 21

Year: 2011 Volume: 20

Year: 2010 Volume: 19

Year: 2009 Volume: 18

Year: 2008 Volume: 17

Year: 2007 Volume: 16

Year: 2006 Volume: 15

Year: 2005 Volume: 14

Year: 2004 Volume: 13

Year: 2003 Volume: 12

Year: 2002 Volume: 11

Year: 2001 Volume: 10

Year: 2000 Volume: 9

Year: 1999 Volume: 8

Year: 1998 Volume: 7

Year: 1997 Volume: 6

Year: 1997 Volume: 6 Issue: 12

Year: 1997 Volume: 6 Issue: 11

Year: 1997 Volume: 6 Issue: 10

Year: 1997 Volume: 6 Issue: 9

Year: 1997 Volume: 6 Issue: 8

Year: 1997 Volume: 6 Issue: 7

Year: 1997 Volume: 6 Issue: 6

The chemistry and enzymology of the type I signal peptidases.

Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k.

The uncharged surface features surrounding the active site of Escherichia coli DsbA are conserved and are implicated in peptide binding.

Structural studies of the streptavidin binding loop.

De novo design of the hydrophobic core of ubiquitin.

MultiCoil: a program for predicting two- and three-stranded coiled coils.

Optimization of the electrostatic interactions between ionized groups and peptide dipoles in proteins.

Refined structure of villin 14T and a detailed comparison with other actin-severing domains.

Identification of compact, hydrophobically stabilized domains and modules containing multiple peptide chains.

Refined structures of three crystal forms of toxic shock syndrome toxin-1 and of a tetramutant with reduced activity.

Characterization of the receptor binding determinants of granulocyte colony stimulating factor.

Secondary and tertiary structural changes in gamma delta resolvase: comparison of the wild-type enzyme, the I110R mutant, and the C-terminal DNA binding domain in solution.

Refined solution structure and backbone dynamics of HIV-1 Nef.

The role of context on alpha-helix stabilization: host-guest analysis in a mixed background peptide model.

Design of a leucine zipper coiled coil stabilized 1.4 kcal mol-1 by phosphorylation of a serine in the e position.

Mapping of the plasminogen binding site of streptokinase with short synthetic peptides.

On the calculation of binding free energies using continuum methods: application to MHC class I protein-peptide interactions.

Hydrophobicity regained.

Determination of the disulfide bond arrangement of human respiratory syncytial virus attachment (G) protein by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Hydrogen exchange properties of proteins in native and denatured states monitored by mass spectrometry and NMR.

The adaptability of Escherichia coli thioredoxin to non-conservative amino acid substitutions.

Automated design of the surface positions of protein helices.

Crystallographic analysis of the pH-dependent binding of iminobiotin by streptavidin.

Characterization and crystallization of human uroporphyrinogen decarboxylase.

Nuclear magnetic resonance assignment and secondary structure of an ankyrin-like repeat-bearing protein: myotrophin.

Regulation and crystallization of phosphorylated and dephosphorylated forms of truncated dimeric phenylalanine hydroxylase.

How protein chemists learned about the hydrophobic factor.

Year: 1997 Volume: 6 Issue: 5

Year: 1997 Volume: 6 Issue: 4

Year: 1997 Volume: 6 Issue: 3

Year: 1997 Volume: 6 Issue: 2

Year: 1997 Volume: 6 Issue: 1

Year: 1996 Volume: 5

Year: 1995 Volume: 4

Year: 1994 Volume: 3

Year: 1993 Volume: 2

Year: 1992 Volume: 1

Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Linse, S. Forsén, S. Andersson, M. Svensson, L. A. Ivarsson, I. Malmendal, A.
Description	Country affiliation: Sweden Author Affiliation: Andersson M ( Department of Molecular Biophysics, Lund University, Sweden.)
Abstract	The three-dimensional structures of the magnesium- and manganese-bound forms of calbindin D9k were determined to 1.6 A and 1.9 A resolution, respectively, using X-ray crystallography. These two structures are nearly identical but deviate significantly from both the calcium bound form and the metal ion-free (apo) form. The largest structural differences are seen in the C-terminal EF-hand, and involve changes in both metal ion coordination and helix packing. The N-terminal calcium binding site is not occupied by any metal ion in the magnesium and manganese structures, and shows little structural deviation from the apo and calcium bound forms. 1H-NMR and UV spectroscopic studies at physiological ion concentrations show that the C-terminal site of the protein is significantly populated by magnesium at resting cell calcium levels, and that there is a negative allosteric interaction between magnesium and calcium binding. Calcium binding was found to occur with positive cooperativity at physiological magnesium concentration.
ISSN	09618368
e-ISSN	1469896X
Journal	Protein Science
Issue Number	6
Volume Number	6
Language	English
Publisher	Wiley-Blackwell (on behalf of The Protein Society)
Publisher Date	1997-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Calcium Chemistry Magnesium S100 Calcium Binding Protein G Allosteric Regulation Calbindins Metabolism Cations, Divalent Crystallography, X-ray Magnetic Resonance Spectroscopy Models, Chemical Models, Molecular Protein Conformation Comparative Study Research Support, Non-u.s. Gov't Discipline Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Biochemistry

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in