NDLI: NMR chemical shift perturbation mapping of DNA binding by a zinc-finger domain from the yeast transcription factor ADR1.

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NMR chemical shift perturbation mapping of DNA binding by a zinc-finger domain from the yeast transcription factor ADR1.

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NMR chemical shift perturbation mapping of DNA binding by a zinc-finger domain from the yeast transcription factor ADR1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rajagopal, P. Klevit, R. E. Schmiedeskamp, M.
Description	Country affiliation: United States Author Affiliation: Schmiedeskamp M ( Biomolecular Structure Center, University of Washington, Seattle 98195, USA.)
Abstract	Mutagenesis studies have revealed that the minimal DNA-binding domain of the yeast transcription factor ADR1 consists of two Cys2-His2 zinc fingers plus an additional 20 residues proximal and N-terminal to the fingers. We have assigned NMR 1H, 15N, and 13C chemical shifts for the entire minimal DNA-binding domain of ADR1 both free and bound to specific DNA. 1H chemical shift values suggest little structural difference between the zinc fingers in this construct and in single-finger constructs, and 13C alpha chemical shift index analysis indicates little change in finger structure upon DNA binding. 1H chemical shift perturbations upon DNA binding are observed, however, and these are mapped to define the protein-DNA interface. The two zinc fingers appear to bind DNA with different orientations, as the entire helix of finger 1 is perturbed, while only the extreme N-terminus of the finger 2 helix is affected. Furthermore, residues N-terminal to the first finger undergo large chemical shift changes upon DNA binding suggesting a role at the protein-DNA interface. A striking correspondence is observed between the protein-DNA interface mapped by chemical shift changes and that previously mapped by mutagenesis.
ISSN	09618368
e-ISSN	1469896X
Journal	Protein Science
Issue Number	9
Volume Number	6
Language	English
Publisher	Wiley-Blackwell (on behalf of The Protein Society)
Publisher Date	1997-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Dna-binding Proteins Chemistry Metabolism Magnetic Resonance Spectroscopy Saccharomyces Cerevisiae Proteins Saccharomyces Cerevisiae Transcription Factors Zinc Fingers Amino Acid Sequence Binding Sites Genetics Escherichia Coli Molecular Sequence Data Mutagenesis Peptide Fragments Peptide Mapping Protein Structure, Secondary Research Support, U.s. Gov't, Non-p.h.s. Research Support, U.s. Gov't, P.h.s. Discipline Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Biochemistry

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