NDLI: A structure for the yeast prohibitin complex: Structure prediction and evidence from chemical crosslinking and mass spectrometry

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A structure for the yeast prohibitin complex: Structure prediction and evidence from chemical crosslinking and mass spectrometry

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	De Koning, Leo J. Van Der Spek, Hans Back, Jaap W. De Jong, Luitzen Grivell, Les A. Sanz, Marta Artal De Koster, Chris G. Muijsers, Anton O. Nijtmans, Leo G. J.
Description	Country affiliation: Netherlands Author Affiliation: Back JW ( Swammerdam Institute for Life Sciences (SILS)-Mass Spectrometry Group, University of Amsterdam, Nieuwe Achtergracht 166, The Netherlands. jwback@science.uva.nl)
Abstract	The mitochondrial prohibitin complex consists of two subunits (PHB1 of 32 kD and PHB2 of 34 kD), assembled into a membrane-associated supercomplex of approximately 1 MD. A chaperone-like function in holding and assembling newly synthesized mitochondrial polypeptide chains has been proposed. To further elucidate the function of this complex, structural information is necessary. In this study we use chemical crosslinking, connecting lysine side chains, which are well scattered along the sequence. Crosslinked peptides from protease digested prohibitin complexes were identified with mass spectrometry. From these results, spatial restraints for possible protein conformation were obtained. Many interaction sites between PHB1 and PHB2 were found, whereas no homodimeric interactions were observed. Secondary and tertiary structural predictions were made using several algorithms and the models best fitting the spatial restraints were selected for further evaluation. From the structure predictions and the crosslink data we derived a structural building block of one PHB1 and one PHB2 subunit, strongly intertwined along most of their length. The size of the complex implies that approximately 14 of these building blocks are present. Each unit contains a putative transmembrane helix in PHB2. Taken together with the unit building block we postulate a circular palisade-like arrangement of the building blocks projecting into the intermembrane space.
ISSN	09618368
e-ISSN	1469896X
Journal	Protein Science
Issue Number	10
Volume Number	11
Language	English
Publisher	Wiley-Blackwell (on behalf of The Protein Society)
Publisher Date	2002-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Proteins Chemistry Repressor Proteins Saccharomyces Cerevisiae Amino Acid Sequence Mass Spectrometry Mitochondria Molecular Sequence Data Protein Structure, Secondary Isolation & Purification Sequence Alignment Research Support, Non-u.s. Gov't Discipline Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Biochemistry

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