NDLI: Identifying Plasmodium falciparum cytoadherence-linked asexual protein 3 (CLAG 3) sequences that specifically bind to C32 cells and erythrocytes

Please wait, while we are loading the content...

Year: 2017 Volume: 26

Year: 2016 Volume: 25

Year: 2015 Volume: 24

Year: 2014 Volume: 23

Year: 2013 Volume: 22

Year: 2012 Volume: 21

Year: 2011 Volume: 20

Year: 2010 Volume: 19

Year: 2009 Volume: 18

Year: 2008 Volume: 17

Year: 2007 Volume: 16

Year: 2006 Volume: 15

Year: 2005 Volume: 14

Year: 2005 Volume: 14 Issue: 12

Year: 2005 Volume: 14 Issue: 11

Year: 2005 Volume: 14 Issue: 10

Year: 2005 Volume: 14 Issue: 9

Year: 2005 Volume: 14 Issue: 8

Year: 2005 Volume: 14 Issue: 7

Year: 2005 Volume: 14 Issue: 6

Year: 2005 Volume: 14 Issue: 5

Year: 2005 Volume: 14 Issue: 4

Year: 2005 Volume: 14 Issue: 3

Year: 2005 Volume: 14 Issue: 2

The H2A.Z/H2B dimer is unstable compared to the dimer containing the major H2A isoform

Descriptor-based protein remote homology identification.

The crystal structures of semi-synthetic aequorins

Sequence swapping does not result in conformation swapping for the beta4/beta5 and beta8/beta9 beta-hairpin turns in human acidic fibroblast growth factor.

NMR structure of HI0004, a putative essential gene product from Haemophilus influenzae, and comparison with the X-ray structure of an Aquifex aeolicus homolog.

Specificity and reactive loop length requirements for crmA inhibition of serine proteases

EDD, a novel phosphotransferase domain common to mannose transporter EIIA, dihydroxyacetone kinase, and DegV.

Crystal structures of RsbQ, a stress-response regulator in Bacillus subtilis.

The mechanism of inhibition of the cyclin-dependent kinase-2 as revealed by the molecular dynamics study on the complex CDK2 with the peptide substrate HHASPRK

Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo

Regulation of protein activity with small-molecule-controlled inteins.

Assessing predictions of protein-protein interaction: the CAPRI experiment.

Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor

Improving the alkalophilic performances of the Xyl1 xylanase from Streptomyces sp. S38: structural comparison and mutational analysis.

Stability of mutant serpin/furin complexes: dependence on pH and regulation at the deacylation step.

Physicochemical and residue conservation calculations to improve the ranking of protein-protein docking solutions.

Stability of HAMLET--a kinetically trapped alpha-lactalbumin oleic acid complex.

An engineered chaperonin caging a guest protein: Structural insights and potential as a protein expression tool.

Spectral magnitude effects on the analyses of secondary structure from circular dichroism spectroscopic data.

Helical structure determined by NMR of the HIV-1 (345–392)Gag sequence, surrounding p2: Implications for particle assembly and RNA packaging

The crystal structure of an eukaryotic iron superoxide dismutase suggests intersubunit cooperation during catalysis.

Crystal structure of the complex formed between a group I phospholipase A2 and a naturally occurring fatty acid at 2.7 A resolution.

Folding and particle assembly are disrupted by single-point mutations near the autocatalytic cleavage site of Nudaurelia capensis omega virus capsid protein.

An avidin-like domain that does not bind biotin is adopted for oligomerization by the extracellular mosaic protein fibropellin.

Deamidation: Differentiation of aspartyl from isoaspartyl products in peptides by electron capture dissociation.

Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum erythrocyte binding ligand 1 (EBL-1)-derived peptides.

Intramolecular disulphide bond arrangements in nonhomologous proteins.

Reversible self-association of ovalbumin at air-water interfaces and the consequences for the exerted surface pressure.

Identifying Plasmodium falciparum cytoadherence-linked asexual protein 3 (CLAG 3) sequences that specifically bind to C32 cells and erythrocytes

Mapping protein energy landscapes with amide hydrogen exchange and mass spectrometry: I. A generalized model for a two-state protein and comparison with experiment.

Year: 2005 Volume: 14 Issue: 1

Year: 2004 Volume: 13

Year: 2003 Volume: 12

Year: 2002 Volume: 11

Year: 2001 Volume: 10

Year: 2000 Volume: 9

Year: 1999 Volume: 8

Year: 1998 Volume: 7

Year: 1997 Volume: 6

Year: 1996 Volume: 5

Year: 1995 Volume: 4

Year: 1994 Volume: 3

Year: 1993 Volume: 2

Year: 1992 Volume: 1

Identifying Plasmodium falciparum cytoadherence-linked asexual protein 3 (CLAG 3) sequences that specifically bind to C32 cells and erythrocytes

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	García, Javier E. Ocampo, Marisol Curtidor, Hernando Puentes, Alvaro Torres, Elizabeth López, Ramses López, Yolanda Valbuena, John J. Patarroyo, Manuel A. Vera, Ricardo Rodríguez, Luis E. Patarroyo, Manuel E. Tovar, Diana Cortes, Jimena Ramírez, Luis E.
Description	Country affiliation: Colombia Author Affiliation: Ocampo M ( Fundación Instituto de Immunologia de Colombia and Universidad Nacional de Colombia, Avda. Calle 26 No. 50-00, Bogotá, Colombia. marisol_ocampo@fidic.org.co)
Abstract	Adhesion of mature asexual stage Plasmodium falciparum parasite-infected erythrocytes (iRBC) to the vascular endothelium is a critical event in the pathology of Plasmodium falciparum malaria. It has been suggested that the clag gene family is essential in cytoadherence to endothelial receptors. Primers used in PCR and RT-PCR assays allowed us to determine that the gene encoding CLAG 3 (GenBank accession no. NP_473155) is transcribed in the Plasmodium falciparum FCB2 strain. Western blot showed that antisera produced against polymerized synthetic peptides from this protein recognized a 142-kDa band in P. falciparum schizont lysate. Seventy-one 20-amino-acid-long nonoverlapping peptides, spanning the CLAG 3 (cytoadherence-linked asexual protein on chromosome 3) sequence were tested in C32 cell and erythrocyte binding assays. Twelve CLAG peptides specifically bound to C32 cells (which mainly express CD36) with high affinity, hereafter referred to as high-affinity binding peptides (HABPs). Five of them also bound to erythrocytes. HABP binding to C32 cells and erythrocytes was independent of peptide charge or peptide structure. Affinity constants were between 100 nM and 800 nM. Cross-linking and SDS-PAGE analysis allowed two erythrocyte binding proteins of around 26 kDa and 59 kDa to be identified, while proteins of around 53 kDa were identified as possible receptor sites for C-32 cells. The HABPs' role in Plasmodium falciparum invasion inhibition was determined. Such an approach analyzing various CLAG 3 regions may elucidate their functions and may help in the search for new antigens important for developing antimalarial vaccines.
ISSN	09618368
e-ISSN	1469896X
Journal	Protein Science
Issue Number	2
Volume Number	14
Language	English
Publisher	Wiley-Blackwell (on behalf of The Protein Society)
Publisher Date	2005-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Adhesion Molecules Chemistry Erythrocytes Metabolism Parasitology Plasmodium Falciparum Protozoan Proteins Physiology Amino Acid Sequence Animals Antibodies Antigens, Cd36 Blotting, Western Circular Dichroism Cross-linking Reagents Dna Primers Dna, Complementary Electrophoresis, Polyacrylamide Gel Immunoblotting Molecular Sequence Data Peptides Polymerase Chain Reaction Polymers Protein Binding Protein Conformation Protein Structure, Tertiary Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Research Support, Non-u.s. Gov't Discipline Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Biochemistry

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in