NDLI: The role of the 132-160 region in prion protein conformational transitions.

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The role of the 132-160 region in prion protein conformational transitions.

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The role of the 132-160 region in prion protein conformational transitions.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Alvarez-Martinez, Maria Teresa Lange, Reinhard Balny, Claude Torrent, Joan Liautard, Jean-Pierre
Description	Country affiliation: France Author Affiliation: Torrent J ( INSERM U710, CC 105, Université de Montpellier 2, Place Eugène Bataillon, F-34095 Montpellier cédex 5, France.)
Abstract	The native conformation of host-encoded cellular prion protein (PrP(C)) is metastable. As a result of a post-translational event, PrP(C) can convert to the scrapie form (PrP(Sc)), which emerges as the essential constituent of infectious prions. Despite thorough research, the mechanism underlying this conformational transition remains unknown. However, several studies have highlighted the importance of the N-terminal region spanning residues 90-154 in PrP folding. In order to understand why PrP folds into two different conformational states exhibiting distinct secondary and tertiary structure, and to gain insight into the involvement of this particular region in PrP transconformation, we studied the pressure-induced unfolding/ refolding of recombinant Syrian hamster PrP expanding from residues 90-231, and compared it with heat unfolding. By using two intrinsic fluorescent variants of this protein (Y150W and F141W), conformational changes confined to the 132-160 segment were monitored. Multiple conformational states of the Trp variants, characterized by their spectroscopic properties (fluorescence and UV absorbance in the fourth derivative mode), were achieved by tuning the experimental conditions of pressure and temperature. Further insight into unexplored conformational states of the prion protein, likely to mimic the in vivo structural change, was obtained from pressure-assisted cold unfolding. Furthermore, salt-induced conformational changes suggested a structural stabilizing role of Tyr150 and Phe141 residues, slowing down the conversion to a beta-sheet form.
ISSN	09618368
e-ISSN	1469896X
Journal	Protein Science
Issue Number	4
Volume Number	14
Language	English
Publisher	Wiley-Blackwell (on behalf of The Protein Society)
Publisher Date	2005-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Prions Chemistry Amino Acids Animals Cricetinae Freezing Mesocricetus Protein Conformation Drug Effects Protein Folding Sodium Chloride Pharmacology Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Research Support, Non-u.s. Gov't Discipline Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Biochemistry

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