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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bujnicki, Janusz M. Pierechod, Marcin Purta, Elzbieta Nowak, Agnieszka Saari, Anna Konieczny, Igor |
| Description | Country affiliation: Poland Author Affiliation: Pierechod M ( Department of Molecular and Cellular Biology, Faculty of Biotechnology, University of Gdansk, Gdansk, Poland.) |
| Abstract | Proteins from the Rep family of DNA replication initiators exist mainly as dimers, but only monomers can initiate DNA replication by interaction with the replication origin (ori). In this study, we investigated both the activation (monomerization) and the degradation of the broad-host-range plasmid RK2 replication initiation protein TrfA, which we found to be a member of a class of DNA replication initiators containing winged helix (WH) domains. Our in vivo and in vitro experiments demonstrated that the ClpX-dependent activation of TrfA leading to replicationally active protein monomers and mutations affecting TrfA dimer formation, result in the inhibition of TrfA protein degradation by the ClpXP proteolytic system. These data revealed that the TrfA monomers and dimers are degraded at substantially different rates. Our data also show that the plasmid replication initiator activity and stability in E. coli cells are affected by ClpXP system only when the protein sustains dimeric form. |
| ISSN | 09618368 |
| e-ISSN | 1469896X |
| DOI | 10.1002/pro.68 |
| Journal | Protein Science |
| Issue Number | 3 |
| Volume Number | 18 |
| Language | English |
| Publisher | Wiley-Blackwell (on behalf of The Protein Society) |
| Publisher Date | 2009-03-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Adenosine Triphosphatases Metabolism Endopeptidase Clp Escherichia Coli Proteins Chemistry Molecular Chaperones Amino Acid Substitution Dna Replication Escherichia Coli Enzymology Genetics Models, Molecular Protein Binding Protein Multimerization Protein Stability Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Biochemistry |
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