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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fenton, Aron W. Johnson, Troy A. Holyoak, Todd |
| Description | Country affiliation: United States Author Affiliation: Fenton AW ( Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, Kansas 66160, USA. afenton@kumc.edu) |
| Abstract | In the study of rabbit muscle pyruvate kinase (M1-PYK), proline has previously been used as an osmolyte in an attempt to determine a role for preexisting conformational equilibria in allosteric regulation. In this context, osmolytes are small molecules assumed to have no direct interaction with the protein. In contrast to proline's proposed role as an osmolyte, the structure of M1PYK-Mn-pyruvate-proline complex reported herein demonstrates that proline binds specifically to the allosteric site of M1-PYK. Therefore, this amino acid is an allosteric effector rather than a benign osmolyte. Other compounds often used as osmolytes (polyethyleneglycol and glycerol) are also present in the structure, suggesting an interaction with the protein that would, in turn, prevent the usefulness of these compounds in the study of this and most likely other proteins. These findings highlight the need to verify that compounds used as osmolytes to perturb preexisting conformational equilibrium do not directly interact with the protein, a consideration not commonly addressed in the past. |
| ISSN | 09618368 |
| e-ISSN | 1469896X |
| DOI | 10.1002/pro.450 |
| Journal | Protein Science |
| Issue Number | 9 |
| Volume Number | 19 |
| Language | English |
| Publisher | Wiley-Blackwell (on behalf of The Protein Society) |
| Publisher Date | 2010-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Proline Metabolism Pyruvate Kinase Chemistry Allosteric Regulation Animals Crystallography, X-ray Models, Molecular Muscles Enzymology Protein Binding Protein Conformation Rabbits Research Support, N.i.h., Extramural Discipline Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Biochemistry |
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