NDLI: Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF.

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Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, Zhe Sprang, Stephen R. Guo, Liang Sternweis, Paul C.
Description	Country affiliation: United States Author Affiliation: Chen Z ( Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA. Zhe.Chen@utsouthwestern.edu)
Abstract	p115-RhoGEF (p115) belongs to the family of RGS-containing guanine nucleotide exchange factors for Rho GTPases (RGS-RhoGEFs) that are activated by G12 class heterotrimeric G protein subunits. All RGS-RhoGEFs possess tandemly linked Dbl-homology (DH) and plekstrin-homology (PH) domains, which bind and catalyze the exchange of GDP for GTP on RhoA. We have identified that the linker region connecting the N-terminal RGS-homology (RH) domain and the DH domain inhibits the intrinsic guanine nucleotide exchange (GEF) activity of p115, and determined the crystal structures of the DH/PH domains in the presence or absence of the inhibitory linker region. An N-terminal extension of the canonical DH domain (the GEF switch), which is critical to GEF activity, is well folded in the crystal structure of DH/PH alone, but becomes disordered in the presence of the linker region. The linker region is completely disordered in the crystal structure and partially disordered in the molecular envelope calculated from measurements of small angle x-ray scattering (SAXS). It is possible that G subunits activate p115 in part by relieving autoinhibition imposed by the linker region.
ISSN	09618368
e-ISSN	1469896X
DOI	10.1002/pro.542
Journal	Protein Science
Issue Number	1
Volume Number	20
Language	English
Publisher	Wiley-Blackwell (on behalf of The Protein Society)
Publisher Date	2011-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Guanine Nucleotide Exchange Factors Chemistry Amino Acid Motifs Crystallography, X-ray Antagonists & Inhibitors Protein Structure, Tertiary Rho Guanine Nucleotide Exchange Factors Scattering, Small Angle Structure-activity Relationship X-ray Diffraction Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s. Discipline Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Biochemistry

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