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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tao, Li Huang, Yunping Russell, Reb J. Rieble, Siegfried Conover, Matthew O'Mara, Brian Ludwig, Richard Grace, Michael J. Fu, Jinmei Li, Zheng Jian |
| Description | Country affiliation: United States Author Affiliation: Huang Y ( Department of Biologics Product & Process Development, Bristol-Myers Squibb Technical Operations, Princeton, New Jersey 08543, USA. yunping.huang@bms.com) |
| Abstract | A novel amino acid misincorporation, in which the intended glycine (Gly) residues were replaced by a glutamic acid (Glu), was observed in a recombinant protein expressed by Escherichia coli. The misincorporation was identified by peptide mapping and liquid chromatography-tandem mass spectrometric analysis on proteolyzed peptides of the protein and verified using the corresponding synthetic peptides containing the misincorporated residues. Analysis of the distribution of the misincorporated residues and their codon usage shows strong correlation between this misincorporation and the use of rarely used codon within the E. coli expression system. Results in this study suggest that the usage of the rare codon GGA has resulted in a Glu for Gly misincorporation. |
| ISSN | 09618368 |
| e-ISSN | 1469896X |
| DOI | 10.1002/pro.2046 |
| Journal | Protein Science |
| Issue Number | 5 |
| Volume Number | 21 |
| Language | English |
| Publisher | Wiley-Blackwell (on behalf of The Protein Society) |
| Publisher Date | 2012-05-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Codon Escherichia Coli Genetics Glutamic Acid Glycine Recombinant Proteins Chemistry Amino Acid Sequence Amino Acid Substitution Metabolism Molecular Sequence Data Peptide Fragments Research Support, Non-u.s. Gov't Discipline Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Biochemistry |
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