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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sen, Pratik Yadav, Rajeev |
| Description | Country affiliation: India Author Affiliation: Yadav R ( Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208 016, Uttar Pradesh, India.) |
| Abstract | This study is devoted to understand the unfolding mechanism of a multidomain protein, human serum albumin (HSA), in absence and presence of the sucrose by steady-state and time-resolved fluorescence spectroscopy with domain specific marker molecules and is further being substantiated by molecular dynamics (MD) simulation. In water, the domain III of HSA found to unfold first followed by domains I and II as the concentration of GnHCl is increased in the medium. The sequential unfolding behavior of different domains of HSA remains same in presence of sucrose; however, a higher GnHCl concentration is required for unfolding, suggesting stabilizing effect of sucrose on HSA. Domain I is found to be most stabilized by sucrose. The stabilization of domain II is somewhat similar to domain I, but the effect of sucrose on domain III is found to be very small. MD simulation also predicted a similar behavior of sucrose on HSA. The stabilizing effect of sucrose is explained in terms of the entrapment of water molecules in between HSA surface and sucrose layer as well as direct interaction between HSA and sucrose. |
| ISSN | 09618368 |
| e-ISSN | 1469896X |
| DOI | 10.1002/pro.2357 |
| Journal | Protein Science |
| Issue Number | 11 |
| Volume Number | 22 |
| Language | English |
| Publisher | Wiley-Blackwell (on behalf of The Protein Society) |
| Publisher Date | 2013-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Protein Unfolding Serum Albumin Chemistry Sucrose Metabolism Anilino Naphthalenesulfonates Animals Circular Dichroism Guanidine Hemin Models, Molecular Molecular Dynamics Simulation Protein Denaturation Protein Stability Protein Structure, Tertiary Spectrometry, Fluorescence Tryptophan Research Support, Non-u.s. Gov't Discipline Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Biochemistry |
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