NDLI: Nicotinic acid modulates intracellular calcium concentration and disassembles the cytoskeleton

Please wait, while we are loading the content...

Year: 2017 Volume: 15

Year: 2016 Volume: 14

Year: 2016 Volume: 13

Year: 2015 Volume: 12

Year: 2015 Volume: 11

Year: 2014 Volume: 10

Year: 2014 Volume: 10 Issue: 6

Increased production of suilysin contributes to invasive infection of the Streptococcus suis strain 05ZYH33

Nicotinic acid modulates intracellular calcium concentration and disassembles the cytoskeleton

Inducing human parthenogenetic embryonic stem cells into isletlike clusters.

Coxsackievirus B detection in cases of myocarditis, myopericarditis, pericarditis and dilated cardiomyopathy in hospitalized patients.

Proteome changes in mesenteric lymph induced by sepsis.

Cucurbitacin B inhibits proliferation and induces apoptosis via STAT3 pathway inhibition in A549 lung cancer cells

A polymorphism site in the premiR34a coding region reduces miR34a expression and promotes osteosarcoma cell proliferation and migration.

Protein-protein interaction analysis of distinct molecular pathways in two subtypes of colorectal carcinoma.

Low-magnitude high-frequency loading, by whole-body vibration, accelerates early implant osseointegration in ovariectomized rats.

Effects of gastrokine2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways.

Tumor cell lysate induces the immunosuppression and apoptosis of mouse immunocytes.

Metformin reverses multidrug resistance in human hepatocellular carcinoma Bel7402/5fluorouracil cells.

Ezetimibe prevents the development of non-alcoholic fatty liver disease induced by high-fat diet in C57BL/6J mice

Insights into the paracrine effects of uterine natural killer cells.

Genistein decreases cellular redox potential, partially suppresses cell growth in HL60 leukemia cells and sensitizes cells to Î³radiationinduced cell death.

Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats

Short hairpin RNA targeting Notch2 inhibits U87 human glioma cell proliferation by inducing cell cycle arrest and apoptosis in vitro and in vivo.

Dgalactoseinduced mitochondrial DNA oxidative damage in the auditory cortex of rats.

Year: 2014 Volume: 10 Issue: 5

Year: 2014 Volume: 10 Issue: 4

Year: 2014 Volume: 10 Issue: 3

Year: 2014 Volume: 10 Issue: 2

Year: 2014 Volume: 10 Issue: 1

Year: 2014 Volume: 9

Year: 2013 Volume: 8

Year: 2013 Volume: 7

Year: 2012 Volume: 6

Year: 2012 Volume: 5

Year: 2011 Volume: 4

Year: 2010 Volume: 3

Year: 2008 Volume: 1

Nicotinic acid modulates intracellular calcium concentration and disassembles the cytoskeleton

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, Penghui Niu, Hua Li, Yanxi Li, Jiejing Shi, Yu
Description	Author Affiliation: Li J ( Department of Clinical Laboratory, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.); Li Y ( Laboratory of Developmental Diseases in Childhood of Education Ministry, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorder, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.); Zhang P ( Department of Clinical Laboratory, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.); Niu H ( Clinical Laboratory Centre, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China.); Shi Y ( Department of Clinical Laboratory, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.)
Abstract	Nicotinic acid (NA), a member of the vitamin B family, is well known for its functions in the treatment and prevention of atherosclerosis due to decreasing plasma levels of low-density lipoprotein cholesterol. In recent years, the major side effect of NA, cutaneous flushing, has also attracted extensive attention. However, the effects of NA in other aspects of physiology or cell biology have remained elusive. The present study provided evidence that high concentrations of NA were able to first reduce and later elevate intracellular [Ca2+] in the NIH3T3 cell line. The reduction of the intracellular Ca2+ concentration was achieved within the initial 10 sec, and was preceded by a gradual elevation of intracellular [Ca2+]. Notably, marked accumulation of opaque materials in the perinuclear region was observed in NIH3T3 cells treated with 70 mM NA. Further analysis revealed that treatment with 70 mM NA for 1 h disassembled the microtubule and Factin cytoskeleton systems and resulted in ßtubulin degradation in an ubiquitinproteasome-dependent manner. These data indicated that high concentrations of NA disrupted cytoskeleton structures, which may have contributed to minus end (nucleus region) to plus end (cell membrane region)-directed transport processes and resulted in the deposition of material in the perinuclear region. Artificially increasing [Ca2+] adding CaCl2 to the culture media effected the disassembly of Factin, while it had no apparent effect on microtubules. These results suggested that the disruption of the cytoskeleton systems was not entirely due to the NA-induced elevation of [Ca2+]. Finally, microinjection of NA into xenopus embryos blocked the transport of melanosomes to the peripheral cellular area. In conclusion, the present study indicated that NA disassembles Factin and microtubule systems, thereby blocking cytoskeleton-dependent intracellular transport.
ISSN	17912997
e-ISSN	17913004
DOI	10.3892/mmr.2014.2576
Journal	Molecular Medicine Reports
Issue Number	6
Volume Number	10
Language	English
Publisher	Spandidos Publications
Publisher Date	2014-12-01
Publisher Place	Greece
Access Restriction	Open
Subject Keyword	Calcium Metabolism Cytoplasm Cytoskeleton Niacin Actins Animals Cho Cells Cells, Cultured Cricetulus Mice Microtubules Nih 3t3 Cells Proteasome Endopeptidase Complex Tubulin Ubiquitin Xenopus Research Support, Non-u.s. Gov't Discipline Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in