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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Monteiro, Alex Willian A. DE Oliveira, Edilamar Menezes Fernandes, Tiago Phillips, M. Ian DA Silva, Natan D. Soci, Ursula P. R. |
| Description | Country affiliation: Brazil Author Affiliation: DA Silva ND ( Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of São Paulo, São Paulo, Brazil.) |
| Abstract | PURPOSE: MicroRNA (miRNA)-126 is angiogenic and has two validated targets: Sprouty-related protein 1 (Spred-1) and phosphoinositol-3 kinase regulatory subunit 2 (PI3KR2), negative regulators of angiogenesis by VEGF pathway inhibition. We investigated the role of swimming training on cardiac miRNA-126 expression related to angiogenesis. METHODS: Female Wistar rats were assigned to three groups: sedentary (S), training 1 (T1, moderate volume), and training 2 (T2, high volume). T1 consisted of 60 min·d of swimming, five times per week for 10 wk with 5% body overload. T2 consisted of the same protocol of T1 until the eighth week; in the ninth week, rats trained for two times a day, and in the 10th week, rats trained for three times a day. MiRNA and PI3KR2 gene expression analysis was performed by real-time polymerase chain reaction in heart muscle. We assessed markers of training, the cardiac capillary-fiber ratio, cardiac protein expression of VEGF, Spred-1, Raf-1/ERK 1/2, and PI3K/Akt/eNOS. RESULTS: The cardiac capillary-fiber ratio increased in T1 (58%) and T2 (101%) compared with S. VEGF protein expression was increased 42% in T1 and 108% in T2. Cardiac miRNA-126 expression increased 26% (T1) and 42% (T2) compared with S, correlated with angiogenesis. The miRNA-126 target Spred-1 protein level decreased 41% (T1) and 39% (T2), which consequently favored an increase in angiogenic signaling pathway Raf-1/ERK 1/2. On the other hand, the gene expression of PI3KR2, the other miRNA-126 target, was reduced 39% (T1) and 78% (T2), and there was an increase in protein expression of components of the PI3K/Akt/eNOS signaling pathway in the trained groups. CONCLUSIONS: This study showed that aerobic training promotes an increase in the expression of miRNA-126 and that this may be related to exercise-induced cardiac angiogenesis, by indirect regulation of the VEGF pathway and direct regulation of its targets that converged in an increase in angiogenic pathways, such as MAPK and PI3K/Akt/eNOS. |
| ISSN | 01959131 |
| Issue Number | 8 |
| Volume Number | 44 |
| e-ISSN | 15300315 |
| Journal | Medicine & Science in Sports & Exercise |
| Language | English |
| Publisher | Lippincott Williams & Wilkins (on behalf of the American College of Sports Medicine) |
| Publisher Date | 2012-08-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Angiogenesis Inducing Agents Metabolism Micrornas Neovascularization, Physiologic Genetics Nerve Tissue Proteins Phosphatidylinositol 3-kinases Vascular Endothelial Growth Factor A Animals Biological Markers Female Gene Expression Myocardium Physical Conditioning, Animal Rats Rats, Wistar Real-time Polymerase Chain Reaction Signal Transduction Swimming Journal Article Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Sports Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Orthopedics and Sports Medicine Physical Therapy, Sports Therapy and Rehabilitation Sports Science |
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