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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kim, Yun Young Kim, Jae Woo Hong, Jung Heum |
| Description | Responsible library: WPRO |
| Abstract | PURPOSE: To investigate the effects of genistein on the survival and production of nitric oxide in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0, 1, and 10 microm genistein using serum-deprived media. Production of nitric oxide and eNOS activity were assessed with the Griess assay and RT-PCR after exposure to genistein for 10 min and one day. Cellular survival was assessed via MTT assay after exposure to genistein for one day. RESULTS: Genistein significantly increased the production of nitric oxide after exposure for 1 min at 10 microm and for 1 day at 1 microm under serum-deprived conditions. Genistein increased eNOS activity and cellular survival in HTMC. CONCLUSIONS: Genistein increases cellular survival under serum-deprived conditions, accompanied with an increase in nitric oxide production after both short-term and long-term exposures. |
| ISSN | 03786471 |
| e-ISSN | 20922374 |
| Journal | Journal of the Korean Ophthalmological Society |
| Issue Number | 8 |
| Volume Number | 52 |
| Language | Korean |
| Publisher | The Korean Ophthalmological Society |
| Publisher Date | 2011-01-01 |
| Publisher Place | Korea (South) |
| Access Restriction | Open |
| Subject Keyword | Original Article Discipline Ophthalmology Discipline Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ophthalmology |
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