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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Park, Kyoung Soo Seong, Gong Je Hong, Sa Min Iizuka, Yoko Kim, Chan Yun |
| Description | Responsible library: WPRO |
| Abstract | PURPOSE: The cytotoxicities and anti-fibrotic effects of mitomycin C and pirfenidone on human dermal fibroblast were evaluated. METHODS: Initially, 24-hour cell cultures were exposed to transforming growth factor (TGF)-beta1, different concentrations of mitomycin C, and pirfenidone solutions in order to evaluate cytotoxicity. Expressions of fibronectin, collagen type 1, alpha smooth muscle, and beta-actin were evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blot in mitomycin C solutions at concentrations of 4 microg/mL and 20 microg/mL, and in pirfenidone solutions at 250 microg/mL and 500 microg/mL. RESULTS: In comparison to cell cultures exposed to TGF-beta1 solutions, cytotoxicities were increased in solutions of mitomycin C at 4 microg/mL, 20 microg/mL, 40 microg/mL and pirfenidone at 500 microg/mL, 750 microg/mL, 1,000 microg/mL (p < 0.05, Mann Whitney U-test). The results of real-time RT-PCR show that expressions of fibronectin, collagen type 1, and alpha smooth muscle were significantly more decreased in all concentrations of mitomycin C and pirfenidone compared to those in TGF-beta1 solution. In western blot analysis, expressions of fibronectin and alpha smooth muscle were decreased in all concentrations of mitomycin C and pirfenidone compared to TGF-beta1 solution. CONCLUSIONS: Both drugs have cytotoxicities and anti-fibrotic effects, but pirfenidone was found to have less cytotoxicity and mitomycin C was found to have more anti-fibrotic effects when compared to each other. |
| ISSN | 03786471 |
| e-ISSN | 20922374 |
| Journal | Journal of the Korean Ophthalmological Society |
| Issue Number | 7 |
| Volume Number | 55 |
| Language | Korean |
| Publisher | The Korean Ophthalmological Society |
| Publisher Date | 2014-01-01 |
| Publisher Place | Korea (South) |
| Access Restriction | Open |
| Subject Keyword | Actins Blotting, Western Cell Culture Techniques Collagen Fibroblasts Fibronectins Mitomycin Muscle, Smooth Transforming Growth Factor Beta1 Transforming Growth Factors Original Article Discipline Ophthalmology Discipline Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ophthalmology |
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