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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Naidu, Ravi Megharaj, Mallavarapu Bahar, Md Mezbaul |
| Description | Country affiliation: Australia Author Affiliation: Bahar MM ( Centre for Environmental Risk Assessment and Remediation (CERAR), University of South Australia, Mawson Lakes Boulevard, Mawson Lakes, SA 5095, Australia) |
| Abstract | A Gram-negative, arsenite-oxidizing bacterial strain, MM-1 tolerant to 20mM arsenite and 200 mM arsenate was isolated from a heavy metal contaminated soil which contained only 8.8 mg kg(-1) of arsenic. Based on 16S rRNA analysis, the strain was closely related to the genus Variovorax. This strain completely oxidized 500 µM of arsenite to arsenate within 3h of incubation in minimal salts medium. Kinetic studies of arsenite oxidation by the cells showed one of the lowest Km (17 µM) and highest Vmax (1.23 × 10(-7) µM min(-1) cell(-1)) values reported to date for whole cell suspension. PCR analysis using degenerate primers confirmed the presence of arsenite oxidase gene and its amino acid sequence was 70-91% identical to the large subunit of most reported arsenite oxidases. The significant arsenite oxidation capacity shown by the strain opens the way to its potential application in arsenic remediation process. |
| ISSN | 03043894 |
| Volume Number | 262 |
| e-ISSN | 18733336 |
| Journal | Journal of Hazardous Materials |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-11-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Betaproteobacteria Enzymology Genetics Oxidoreductases Soil Microbiology Arsenic Chemistry Arsenites Biodegradation, Environmental Cloning, Molecular Dna Primers Genes, Bacterial Kinetics Oxidation-reduction Oxygen Phylogeny Polymerase Chain Reaction Rna, Ribosomal, 16s Sequence Analysis, Dna Soil Soil Pollutants Analysis Journal Article Research Support, Non-u.s. Gov't Discipline Environmental Science Discipline Environmental Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Environmental Chemistry Pollution Waste Management and Disposal Health, Toxicology and Mutagenesis Environmental Engineering |
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