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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Polcaro, Chiara M. Ciccioli, Piero Donati, Enrica Galli, Emanuela |
| Description | Country affiliation: Italy Author Affiliation: Donati E ( Institute of Chemical Methodologies of CNR (IMC), Research Area of Rome 1, Via Salaria Km 29,300, 00015 Monterotondo, Rome, Italy.); Polcaro CM ( Institute of Chemical Methodologies of CNR (IMC), Research Area of Rome 1, Via Salaria Km 29,300, 00015 Monterotondo, Rome, Italy. Electronic address: chiara.polcaro@cnr.it.); Ciccioli P ( Institute of Chemical Methodologies of CNR (IMC), Research Area of Rome 1, Via Salaria Km 29,300, 00015 Monterotondo, Rome, Italy.); Galli E ( Institute of Agro-Environmental and Forest Biology of CNR (IBAF), Research Area of Rome 1, Via Salaria Km 29,300, 00015 Monterotondo, Rome, Italy.) |
| Abstract | For the first time a laccase from Trametes versicolor was immobilized on a natural clinoptilolite with Si/Al=5 to obtain a biocatalyst for environmental applications. Immobilization procedures exploiting adsorption and covalent binding were both tested, and only the last provided enough activity for practical applications. The optimal conditions for the immobilization of the enzyme on the support and the kinetic parameters for the free and covalent bonded laccase were determined. The laccase bonded to the zeolitic support showed a lower activity than the free laccase, but the pH and thermal stability were greater. 20 mg of dry biocatalyst containing 1 U of laccase were able to remove in 50h 73-78% of 2-chlorophenol and 2,4-dichlorophenol in relatively concentrated aqueous solutions (100 µmol L(-1)). |
| ISSN | 03043894 |
| Volume Number | 289 |
| e-ISSN | 18733336 |
| Journal | Journal of Hazardous Materials |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-05-30 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Laccase Chemistry Zeolites Adsorption Catalysis Chlorophenols Enzymes, Immobilized Hydrogen-ion Concentration Kinetics Temperature Trametes Enzymology Comparative Study Journal Article Discipline Environmental Science Discipline Environmental Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Environmental Chemistry Pollution Waste Management and Disposal Health, Toxicology and Mutagenesis Environmental Engineering |
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