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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Frankovic, Vida Brne, Peter Strancar, Ales Podgornik, Ales Lendero, Nika Vidic, Jana |
| Description | Country affiliation: Slovenia Author Affiliation: Lendero N ( BIA Separations d.o.o., Teslova 30, Ljubljana, Slovenia.) |
| Abstract | New non-destructive method for characterization of ion exchange chromatographic columns based on transient pH formed by a step change in ionic strength of buffer solutions was examined. The method was used to distinguish between cation and anion or weak and strong ion exchange chromatographic supports and to determine the capacity of the chromatographic resins. The general scheme to distinguish between most commonly used types of ion exchange chromatographic columns was proposed. The duration of pH transient was shown to be linearly proportional to the total ionic capacity and was used to estimate protein dynamic binding capacity of the resin. The effects of pH, concentration and temperature on transient pH duration were examined. |
| ISSN | 00219673 |
| Issue Number | 1 |
| Journal | Journal of Chromatography A |
| Volume Number | 1185 |
| e-ISSN | 18733778 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-03-21 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Hydrogen-ion Concentration Ion Exchange Resins Chromatography, Ion Exchange Ion Exchange Water Chemistry Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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