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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Segura-Carretero, Antonio Cifuentes, Alejandro Dinelli, Giovanni León, Carlos Fernández-Gutiérrez, Alberto Garcia-Cañas, Virginia García-Villalba, Rocio |
| Description | Country affiliation: Spain Author Affiliation: García-Villalba R ( Faculty of Sciences, University of Granada, Fuentenueva s/n, Granada, Spain.) |
| Abstract | In this work, capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF-MS) is proposed to identify and quantify the main metabolites found in transgenic soybean and its corresponding non-transgenic parental line both grown under identical conditions. The procedure includes optimization of metabolites extraction, separation by CE, on-line electrospray-TOF-MS analysis and data evaluation. A large number of extraction procedures and background electrolytes are tested in order to obtain a highly reproducible and sensitive analytical methodology. Using this approach, a large number of metabolites were tentatively identified based on the high mass accuracy provided by TOF-MS analyzer, together with the isotopic pattern and expected electrophoretic mobility of these compounds. In general, the same metabolites and in similar amounts were found in the conventional and transgenic variety. However, significant differences were also observed in some specific cases when the conventional variety was compared with its corresponding transgenic line. The selection of these metabolites as possible biomarkers of transgenic soybean is discussed, although a larger number of samples need to be analyzed in order to validate this point. It is concluded that metabolomic procedures based on CE-MS can open new perspectives in the study of transgenic foods in order to corroborate (or not) the equivalence with their conventional counterparts. |
| ISSN | 00219673 |
| Issue Number | 1-2 |
| Volume Number | 1195 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-06-27 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Electrophoresis, Capillary Methods Plants, Genetically Modified Metabolism Soybeans Spectrometry, Mass, Electrospray Ionization Plant Preparations Analysis Chemistry Isolation & Purification Genetics Comparative Study Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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