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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kang, Jianzheng Liu, Shaorong Wang, Xiayan Lu, Joann J. Wang, Shili |
| Description | Country affiliation: United States Author Affiliation: Wang X ( Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.) |
| Abstract | We report a unique property of nanocapillaries for chromatographic separations of ionic species. Due to the electric double layer overlap, ions are unevenly distributed inside a nanochannel, with counterions enriched near the wall and co-ions concentrated in the middle of the channel. As a pressure-driven flow is induced, the co-ions will move faster than the counterions. This differential transport results in a chromatographic separation. In this work, we introduce the fundamental mechanism of this separation technology and demonstrate its application for DNA separations. An outstanding feature of this technique is that each separation consumes less than 1 pL sample and generates less than 0.1 nL waste. We also apply this technique for separations of DNA molecules, and efficiencies of more than 1,00000 plates per meter are obtained. |
| ISSN | 00219673 |
| e-ISSN | 18733778 |
| DOI | 10.1016/j.chroma.2008.05.088 |
| Journal | Journal of Chromatography A |
| Issue Number | 2 |
| Volume Number | 1200 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-07-25 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Chromatography Nanotechnology Chemistry Isolation & Purification Models, Theoretical Reproducibility Of Results Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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