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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhao, Dao-Yuan Zhao, Wei-Jun Yang, Ming-Min Zhou, Zhi-Ming Wang, Jing |
| Description | Country affiliation: China Author Affiliation: Zhou ZM ( College of Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.) |
| Abstract | Isoniazid (INH) reacted with p-dimethylaminobenzaldehyde (DABD) in the presence of trichloroacetic acid to give isonicotinylhydrazone (INZ) having lambda(max) 365nm. Cloud point extraction (CPE) is carried out to extract INH and IHZ in aqueous solutions using surfactant poly(ethylene glycol) 4000 (PEG4000), respectively. Langmuir model is used to study the adsorption behaviors of the two solutes on micelles of PEG4000. A linear correlation is found between variation of PEG4000 concentration required for feed concentration of the two solutes and used to predict PEG4000 concentration required for extracting INH and IHZ in CPE procedure. The results calculated show that, for a desired recovery level of 90%, only can IHZ be sufficiently extracted by PEG4000. In this experiment, the feed concentration of PEG4000 is defined by above-mentioned correlation, and the effects of other operating parameters, e.g., concentration of salt, pH and centrifugation time on extraction of PEG4000-IHZ system have also been studied in detail. The proposed CPE method coupled with HPLC-UV system is successfully used for the determination of INH in urine sample. |
| ISSN | 00219673 |
| Issue Number | 1 |
| Volume Number | 1216 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-01-02 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Analytic Sample Preparation Methods Antitubercular Agents Chemistry Isoniazid Urine Benzaldehydes Chromatography, High Pressure Liquid Humans Hydrogen-ion Concentration Polyethylene Glycols Reproducibility Of Results Salts Sensitivity And Specificity Surface-active Agents Tuberculosis Journal Article Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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