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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ramaswamy, Senthilkumar Leahy, Anne Chung, Franklin Asher, Damon Iyer, Ganesh Mehta, Ushma Cheng, Kwok-Shun |
| Description | Country affiliation: United States Author Affiliation: Iyer G ( EMD Millipore, Purification Product Development, Bedford, MA 01730, USA. ganesh.iyer@merckgroup.com) |
| Abstract | A method for flow-through purification of viruses and virus like nano-particles using a combination of binding and size-exclusion chromatography was developed. This technique relies on minimizing the external surface area per unit volume available for virus binding by increasing the mean diameter of the beads used in the column. At the same time the impurity binding capacity of the column is maximized by utilizing beads with multiple functionalities of the optimum size. Purification of different types of viruses and virus-like-particles could be achieved using this technique. Flow-through purification of influenza virus using this technique yielded virus recoveries greater than 70-80% coupled with impurity removal greater than 80%. Finally an approach to optimize and facilitate process development using this technology is presented. Since the impurity binding occurs via a non-specific mechanism and virus recovery is achieved through reduced surface area, the technique is not limited to specific types of viruses and offers the potential as a universal purification tool. |
| ISSN | 00219673 |
| Issue Number | 26 |
| Volume Number | 1218 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-07-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chromatography, Gel Methods Chromatography, Ion Exchange Virion Isolation & Purification Virus Cultivation Viruses Humans Microspheres Nanoparticles Particle Size Journal Article Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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