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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Gruber, Michael A. Waldhier, Magdalena C. Dettmer, Katja Oefner, Peter J. Nürnberger, Nadine Almstetter, Martin F. |
| Description | Country affiliation: Germany Author Affiliation: Waldhier MC ( Institute of Functional Genomics, University of Regensburg, Regensburg, Germany. magdalena.waldhier@klinik.uni-regensburg.de) |
| Abstract | The potential of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS) in the quantitative analysis of amino acid enantiomers (AAEs) as their methyl chloroformate (MCF) derivatives in physiological fluids was investigated. Of the two column sets tested, the combination of an Rt-γDEXsa chiral column with a polar ZB-AAA column provided superior selectivity. Twenty AAEs were baseline resolved including L-Leu and D-Ile, which had failed separation by one-dimensional chiral GC-quadrupole-MS (GC-qMS). Lower limits of quantification (LLOQ) were in the range of 0.03-2 µM. Reproducibility of the analysis of a serum specimen in octaplicate ranged from 1.3 to 16.6%. The GC×GC-TOFMS method was validated by analyzing AAEs in 48 urine and 43 serum specimens, respectively, and by comparing the results with data obtained by a previously validated GC-qMS method. Mean recoveries ranged from 78.4% for D-Leu to 116.4% for D-Pro in urine and 72.2% for L-Thr to 129.4% for L-Ile in serum. The method was applied to the comparison of AAE serum levels in patients suffering from liver cirrhosis to a control group. Significantly increased D-AA concentrations were found for the patient group, whereas L-AA levels were slightly decreased. |
| ISSN | 00219673 |
| Issue Number | 28 |
| Volume Number | 1218 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-07-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Amino Acids Blood Urine Gas Chromatography-mass Spectrometry Methods Metabolomics Chemistry Humans Linear Models Liver Cirrhosis Metabolism Metabolome Reproducibility Of Results Stereoisomerism Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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