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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Cottet, Hervé Destoumieux-Garzón, Delphine Oukacine, Farid Quirino, Joselito P. |
| Description | Country affiliation: France Author Affiliation: Oukacine F ( Institut des Biomolécules Max Mousseron (UMR 5247 CNRS, Université de Montpellier 1, Université de Montpellier 2), place Eugène Bataillon CC 1706, 34095 Montpellier Cedex 5, France.) |
| Abstract | The isotachophoretic analysis of different bacterial strains was studied using capillaries with different internal diameters from 50 to 250 µm. Several injection modes were investigated and compared in order to improve the limit of detection of bacteria by capillary isotachophoresis. A system suitability test obtained from the separation voltage was developed to ensure reliable results. As expected, the use of wider bore capillaries improved the analytical sensitivity of the isotachophoretic method when compared to the 50 µm capillary. With the optimized conditions, the isotachophoretic method presented in this work allows the quantification of Erwinia carotovora (Gram negative bacteria) with a limit of detection as low as ~3000 cells mL(-1). The proposed methodology does not require any additive in the electrolyte such a fluorescent or chromophoric dye to reach these limits of detection. |
| ISSN | 00219673 |
| Journal | Journal of Chromatography A |
| Volume Number | 1268 |
| e-ISSN | 18733778 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-12-14 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacteria Chemistry Electrophoresis, Capillary Methods Isotachophoresis Instrumentation Pectobacterium Carotovorum Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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