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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Buckenmaier, Stephan M. C. McCalley, David V. Fallas, Morgane M. Tanaka, Nobuo |
| Description | Country affiliation: United kingdom Author Affiliation: Fallas MM ( Centre for Research in Biosciences, University of the West of England, Frenchay, Bristol BS16 1QY, UK.) |
| Abstract | The influence of pressure on the retention of several types of solute, including acids, bases and neutrals, was studied by the use of restriction capillaries added to the end of various monomeric and polymeric octadecylsilyl-modified 5µm particle size columns. Although it appeared that certain polymeric columns could give somewhat greater increases in retention with pressure, differences in behaviour between these different C18 columns were rather small. Differences in solute molecular size were most important in determining increases in retention with pressure. However, solute structure such as polarity and planarity were also influential. A prototype C30 column gave interesting selectivity changes between planar and non-planar solutes as a function of pressure. Considerable selectivity differences with pressure were shown when diverse mixtures of solutes were analysed. For the solutes studied, only minor effects of increased pressure on column efficiency and peak shape were noted. |
| ISSN | 00219673 |
| Volume Number | 1297 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-07-05 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chromatography, High Pressure Liquid Instrumentation Methods Chromatography, Reverse-phase Models, Theoretical Solvents Chemistry Acetonitriles Hydrogen-ion Concentration Hydrophobic And Hydrophilic Interactions Molecular Weight Particle Size Pressure Temperature Journal Article Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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