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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hasse, Hans Werner, Albert |
| Description | Country affiliation: Germany Author Affiliation: Werner A ( Laboratory of Engineering Thermodynamics, University of Kaiserslautern, Kaiserslautern, Germany.) |
| Abstract | The influence of mixed electrolytes on the adsorption of macromolecules on a hydrophobic resin is explored. The macromolecules are native lysozyme, di-PEG-lysozyme-5kDa and pure PEG-6kDa, the adsorbent is Toyopearl PPG-600M, a mildly hydrophobic resin. The solvent is a 25 mM aqueous sodium phosphate buffer at pH 7.0 containing additional salts with an overall ionic strength of 3000 mM. The studied salts are sodium chloride, ammonium sulfate, sodium sulfate and ammonium chloride. Both pure salts as well as binary and ternary mixtures of these salts with varying ratios of the amounts of the salts are studied. For all solvents adsorption equilibrium isotherms are measured at 25°C. Mixing electrolytes can lead to synergetic effects, i.e. the adsorption in the mixed electrolyte system is sometimes distinctly larger than expected from the results of the single electrolyte systems. The single ions, not the salts, drive the adsorption. A mathematical model is developed, which describes the influence of the different ions and their cross-interactions on the studied adsorption process. |
| ISSN | 00219673 |
| Volume Number | 1315 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-11-08 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chromatography, Liquid Instrumentation Electrolytes Chemistry Models, Chemical Adsorption Ammonium Sulfate Animals Chickens Methods Hydrophobic And Hydrophilic Interactions Muramidase Metabolism Polyethylene Glycols Sodium Chloride Journal Article Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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