| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Zhao, Wei-Wei Huang, Hongliang Li, Jian-Rong Wang, Xiayan Xie, Yabo Bai, Li-Ping Zhou, You-Ya Yan, Zeng-Guang Li, Fa-Sheng Zhang, Chao-Yan |
| Description |
Author Affiliation: Zhao WW ( Beijing Key Laboratory for Green Catalysis and Separation and Department of Chemistry and Chemical Engineering, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China); Zhang CY ( State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China.); Yan ZG ( State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China.); Bai LP ( State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China.); Wang X ( Beijing Key Laboratory for Green Catalysis and Separation and Department of Chemistry and Chemical Engineering, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China.); Huang H ( Beijing Key Laboratory for Green Catalysis and Separation and Department of Chemistry and Chemical Engineering, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China.); Zhou YY ( State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China. Electronic address: zhouyy@craes.org.cn.); Xie Y ( Beijing Key Laboratory for Green Catalysis and Separation and Department of Chemistry and Chemical Engineering, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China.); Li FS ( State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China.); Li JR ( Beijing Key Laboratory for Green Catalysis and Separation and Department of Chemistry and Chemical Engineering, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China. Electronic address: jrli@bjut.edu.cn.) |
| Abstract |
Metal-organic frameworks (MOFs) have great potential for applications in chromatography due to their highly tailorable porous structures and unique properties. In this work, the stable MOF UiO-66 was evaluated as both a normal-phase (NP-) and a reverse-phase (RP-) stationary phase in the high performance liquid chromatography (HPLC) to separate substituted benzenes (SBs) and polycyclic aromatic hydrocarbons (PAHs). It was found that the mobile phase composition has a significant effect on the HPLC separation. Baseline RP-HPLC separations of xylene isomers; naphthalene and anthracene; naphthalene and chrysene; and naphthalene, fluorene, and chrysene were achieved using MeOH/H2O ratios of 80:20, 75:25, 85:15, and 75:25, respectively, on the UiO-66 column. Similarly, baseline NP-HPLC separations of xylene isomers and ethylbenzene; ethylbenzene, styrene, o-xylene, and m-xylene; and several PAHs were also obtained on the UiO-66 column with different mobile phase compositions. The relative standard deviations (RSDs) of retention time, peak height, peak area, and half peak width for five replicate separations of the tested analytes were within the ranges 0.2-0.4%, 0.2-1.6%, 0.7-3.9%, 0.4-1.1%, respectively. We also evaluated other critical HPLC parameters, including injected sample mass, column temperature, and the thermodynamic characters of both the RP-HPLC and the NP-HPLC separation processes. It was confirmed that the separation of SBs on a UiO-66 column was an exothermic process, controlled by both enthalpy change (ΔH) and entropy change (ΔS). The reverse shape selectivity, size selectivity, stacking effect, and electrostatic force played vital roles in the separations of these analytes. To the best of our knowledge, this method is one of the very few examples of using MOFs as the stationary phase in both NP-HPLC and RP-HPLC. MOF-based stationary phases may thus be applied in the separations and analyses of SBs and PAHs in environmental samples. |
| ISSN |
00219673 |
| Volume Number |
1370 |
| e-ISSN |
18733778 |
| Journal |
Journal of Chromatography A |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-11-28 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Benzene Isolation & Purification Chromatography, High Pressure Liquid Methods Chromatography, Reverse-phase Polycyclic Hydrocarbons, Aromatic Instrumentation Isomerism Microscopy, Electrochemical, Scanning Nanoparticles Ultrastructure Porosity Temperature Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Organic Chemistry Medicine Analytical Chemistry Biochemistry |
