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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lämmerhofer, Michael Moravcová, Dana Planeta, Josef Carrasco-Correa, Enrique Javier Wiedmer, Susanne K. |
| Description | Author Affiliation: Moravcová D ( Institute of Analytical Chemistry of the ASCR, v. v. i., Veverí 97, 60200 Brno, Czech Republic. Electronic address: moravcova@iach.cz.); Carrasco-Correa EJ ( Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.); Planeta J ( Institute of Analytical Chemistry of the ASCR, v. v. i., Veverí 97, 60200 Brno, Czech Republic.); Lämmerhofer M ( Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8, 72076 Tübingen, Germany.); Wiedmer SK ( Department of Chemistry, P.O. Box 55, 00014 University of Helsinki, Finland. Electronic address: susanne.wiedmer@helsinki.fi.) |
| Abstract | In this study a strategy to immobilize phospholipids onto a polymer-based stationary phase is described. Methacrylate-based monoliths in capillary format (150×0.1mm) were modified by soybean phosphatidylcholine through 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide coupling to obtain stationary phases suitable to mimic cell surface membranes. The covalent coupling reaction involves the phosphate group in phospholipids; therefore, the described methodology is suitable for all types of phospholipids. Immobilization of soy bean phosphatidylcholine on the monolith was confirmed by attenuated total reflectance Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry of the fatty alcohol profile, generated upon reductive cleavage of the fatty acyl side chains of the phospholipid on the monolith surface with lithium aluminium hydride. The prepared stationary phases were evaluated through studies on the retention of low-molar mass model analytes including neutral, acidic, and basic compounds. Liquid chromatographic studies confirmed predominant hydrophobic interactions between the analytes and the synthesized stationary phase; however, electrostatic interactions contributed to the retention as well. The synthesized columns showed high stability even with fully aqueous mobile phases such as Dulbecco's phosphate-buffered saline solution. |
| ISSN | 00219673 |
| Volume Number | 1402 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-07-10 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chemistry Techniques, Analytical Instrumentation Methods Chromatography, Liquid Methacrylates Chemistry Phosphatidylcholines Biomimetics Ethyldimethylaminopropyl Carbodiimide Hydrophobic And Hydrophilic Interactions Phospholipids Polymers Water Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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