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Predicative Factors for Corneal Endothelial Cell Migration.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mehta, Jodhbir Singh Peh, Gary Seah, Xin Yi Adnan, Khadijah Soh, Yu Qiang George, Benjamin Lawrence Primalani, Nishal Kishinchand
Description	Country affiliation: Singapore Author Affiliation: Soh YQ ( Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore 2Singapore National Eye Centre, Singapore.); Peh G ( Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore 3Ophthalmology Academic Clinical Program, Duke-National University of Singapore Graduate Medical School, Singapore.); George BL ( Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore.); Seah XY ( Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore.); Primalani NK ( Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore.); Adnan K ( Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore.); Mehta JS ( Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore 2Singapore National Eye Centre, Singapore 3Ophthalmology Academic Clinical Program, Duke-National University of Singapore Graduate Medical School, Singapore 4Department of.)
Abstract	PURPOSE: To characterize the effects of Descemet's stripping, Rho-associated protein kinase inhibitor Y-27632, and donor age on endothelial migration in human corneas maintained in ex vivo culture. METHODS: Twenty-eight cadaveric human corneas underwent ex vivo culture in either standard or Y-27632-supplemented culture medium for 14 days. The posterior surface of each cornea was manipulated to create two types of wounds: scratched wound--corneal endothelial cells (CECs) were denuded from the Descemet's membrane (DM) to leave behind a bare but intact DM; and peeled wound--both the DM and overlying CECs were stripped to leave behind bare corneal stroma. Endothelial migration was assessed via Trypan blue staining. Morphologic traits of CECs were assessed via Alizarin red microscopy and scanning electron microscopy. RESULTS: The CECs migrated preferentially over scratched wounds compared with peeled wounds. Y-27632 supplementation accelerated endothelial migration over scratched wounds. Endothelial migration decreased with advanced donor age for both wound types, regardless of exposure to Y-27632. Y-27632 supplementation resulted in a less rapid decline in endothelial migration for donors older than 50 years of age for scratched surfaces. Greater cell density and hexagonality was observed over scratched wounds compared with peeled wounds, regardless of Y-27632 supplementation. CONCLUSIONS: The presence of an intact DM, Y-27632 supplementation, and young donor age are factors that promote endothelial migration in an ex vivo human cornea culture model. The negative effect of age on endothelial migration can be mitigated by the presence of an intact DM and Y-27632 supplementation.
ISSN	01460404
e-ISSN	15525783
Journal	Investigative Opthalmology & Visual Science
Issue Number	2
Volume Number	57
Language	English
Publisher	Association for Research in Vision and Ophthalmology
Publisher Date	2016-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Movement Physiology Endothelium, Corneal Cytology Amides Pharmacology Anthraquinones Administration & Dosage Cell Count Cell Shape Coloring Agents Culture Media Descemet Membrane Surgery Drug Effects Enzyme Inhibitors Microscopy, Electron, Scanning Organ Culture Techniques Pyridines Staining And Labeling Tissue Donors Trypan Blue Rho-associated Kinases Antagonists & Inhibitors Research Support, Non-u.s. Gov't Discipline Ophthalmology
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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