NDLI: An efficient method for DNA extraction from Cladosporioid fungi.

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An efficient method for DNA extraction from Cladosporioid fungi.

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An efficient method for DNA extraction from Cladosporioid fungi.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Moslem, M. A. Wit, P. J. G. M. Bahkali, A. H. Abd-Elsalam, K. A.
Description	Country affiliation: Saudi Arabia Author Affiliation: Moslem MA ( College of Science, Botany and Microbiology Department, King Saud University, Riyadh, Saudi Arabia.)
Abstract	We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells. In order to overcome this we grew these fungi for three days on agar plates and extracted DNA from mycelium mats after manual or electric homogenization. High-quality DNA was isolated, with an A(260)/A(280) ratio ranging between 1.6 and 2.0. Isolated genomic DNA was efficiently digested with restriction enzymes and produced distinct banding patterns on agarose gels for the different Cladosporium species. Clear DNA fragments from the isolated DNA were amplified by PCR using small and large subunit rDNA primers, demonstrating that this method provides DNA of sufficiently high quality for molecular analyses.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	4
Volume Number	9
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Date	2010-11-23
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Cladosporium Genetics Dna, Fungal Isolation & Purification Classification Dna Primers Dna, Ribosomal Electrophoresis, Agar Gel Phylogeny Polymerase Chain Reaction Species Specificity Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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