NDLI: Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata).

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Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata).

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Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata).

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Khajudparn, P. Prajongjai, T. Tantasawat, P. A. Poolsawat, O.
Description	Country affiliation: Thailand Author Affiliation: Khajudparn P ( School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.)
Abstract	Mungbean improvement via hybridization requires the identification of true F(1) hybrids from controlled crosses before further generations of selfing/crossing and selection. We utilized inter-simple sequence repeat (ISSR) markers for identifying putative F(1) hybrids from six cross combinations whose morphological characteristics were very similar to those of their respective female parents and could not be visually discriminated from the self-pollinated progeny. Based on 10 ISSR primers, polymorphisms were found between female and male parents of all six cross combinations. The highest value of genetic differentiation (21.4%) was found between male and female parents of the SUT3 x M5-1 cross. These 10 ISSR primers gave 2.8-25.0% polymorphism between male and female parents, with a mean of 12.1%, and 0-13.0% polymorphism between F(1) hybrid and female parents, with a mean of 4.8%. F(1) hybrids of all six cross combinations could be differentiated from the self-pollinated progeny of their female parents by using only either ISSR 841 or 857 primers, together with the ISSR 835 primer. We conclude that ISSR markers are useful and efficient for identifying mungbean F(1) hybrids in controlled crosses from different genetic background.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	3
Volume Number	11
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Date	2012-09-17
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Crosses, Genetic Fabaceae Genetics Hybridization, Genetic Microsatellite Repeats Dna, Plant Genetic Markers Reproducibility Of Results Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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