NDLI: Engineering the metabolism of the phenylurea herbicide chlortoluron in genetically modified Arabidopsis thaliana plants expressing the mammalian cytochrome P450 enzyme CYP1A2.

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Engineering the metabolism of the phenylurea herbicide chlortoluron in genetically modified Arabidopsis thaliana plants expressing the mammalian cytochrome P450 enzyme CYP1A2.

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Engineering the metabolism of the phenylurea herbicide chlortoluron in genetically modified Arabidopsis thaliana plants expressing the mammalian cytochrome P450 enzyme CYP1A2.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kebeish, Rashad El-Basheer, Radwa Peterhaensel, Christoph Azab, Ehab
Description	Author Affiliation: Kebeish R ( Plant Biotechnology Laboratory (PBL), Botany Department, Faculty of Science, Zagazig University, El-Gamaa Street, 44519, Zagazig, Egypt, rkebeish@zu.edu.eg.)
Abstract	Transgenic Arabidopsis thaliana plants were generated by introduction of the human P450 CYP1A2 gene, which metabolizes a number of herbicides, insecticides and industrial chemicals. Transgenic A. thaliana plants expressing CYP1A2 gene showed remarkable resistance to the phenylurea herbicide chlortoluron (CTU) supplemented either in plant growth medium or sprayed on foliar parts of the plants. HPLC analyses showed a strong reduction in CTU accumulation in planta supporting the tolerance of transgenic lines to high concentrations of CTU. Besides increased herbicide tolerance, expression of CYP1A2 resulted in no other visible phenotype in transgenic plants. Our data indicate that CYP1A2 can be used as a selectable marker for plant transformation, allowing efficient selection of transgenic lines in growth medium and/or in soil-grown plants. Moreover, these transgenic plants appear to be useful for herbicide resistance as well as phytoremediation of environmental contaminants.
ISSN	09441344
Issue Number	13
Volume Number	21
e-ISSN	16147499
Journal	Environmental Science and Pollution Research
Language	English
Publisher	Springer
Publisher Date	2014-01-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arabidopsis Metabolism Cytochrome P-450 Cyp1a2 Herbicides Phenylurea Compounds Plants, Genetically Modified Agrobacterium Animals Toxicity Biodegradation, Environmental Blotting, Western Chromatography, High Pressure Liquid Genetics Genetic Engineering Administration & Dosage Humans Plant Development Drug Effects Real-time Polymerase Chain Reaction Soil Transformation, Genetic Journal Article Research Support, Non-u.s. Gov't Discipline Environmental Science Discipline Environmental Chemistry
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine

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Engineering the metabolism of the phenylurea herbicide chlortoluron in genetically modified Arabidopsis thaliana plants expressing the mammalian cytochrome P450 enzyme CYP1A2

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Engineering the metabolism of the phenylurea herbicide chlortoluron in genetically modified Arabidopsis thaliana plants expressing the mammalian cytochrome P450 enzyme CYP1A2.

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