NDLI: Molecular detection of Bartonella quintana, B. koehlerae, B. henselae, B. clarridgeiae, Rickettsia felis, and Wolbachia pipientis in cat fleas, France.

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Molecular detection of Bartonella quintana, B. koehlerae, B. henselae, B. clarridgeiae, Rickettsia felis, and Wolbachia pipientis in cat fleas, France.

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Year: 2003 Volume: 9 Issue: 2

Year: 2003 Volume: 9 Issue: 1

Molecular detection of Bartonella quintana, B. koehlerae, B. henselae, B. clarridgeiae, Rickettsia felis, and Wolbachia pipientis in cat fleas, France.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Davoust, Bernard Franc, Michel Raoult, Didier Rolain, Jean-Marc
Spatial Coverage	France
Description	Country affiliation: France Author Affiliation: Rolain JM ( Université de la Méditerranée, Marseille, France.)
Abstract	The prevalences of Bartonella, Rickettsia, and Wolbachia were investigated in 309 cat fleas from France by polymerase chain reaction (PCR) assay and sequencing with primers derived from the gltA gene for Rickettsia, the its and pap31 genes for Bartonella, and the 16S rRNA gene for Anaplasmataceae. Positive PCR results were confirmed by using the Lightcycler and specific primers for the rOmpB of Rickettsia and gltA of Bartonella. R. felis was detected in 25 fleas (8.1%), W. pipientis, an insect symbiont, in 55 (17.8%), and Bartonella in 81 (26.2%), including B. henselae (9/81; 11.1%), B. clarridgeiae (55/81; 67.9%), B. quintana (14/81; 17.3%), and B. koehlerae (3/81; 3.7%). This is the first report of the amplification of B. quintana from fleas and the first description of B. koehlerae in fleas from an area outside the United States. Cat fleas may be more important vectors of human diseases than previously reported.
ISSN	10806040
e-ISSN	10806059
Journal	Emerging Infectious Diseases
Issue Number	3
Volume Number	9
Language	English
Publisher	Centers for Disease Control and Prevention
Publisher Date	2003-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bartonella Genetics Rickettsia Felis Siphonaptera Microbiology Wolbachia Animals Isolation & Purification Dna Primers Polymerase Chain Reaction Discipline Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Epidemiology Microbiology (medical)

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Molecular detection of Bartonella quintana, B-koehlerae, B-henselae, B-clarridgeiae, Rickettsia felis, and Wolbachia pipientis in cat fleas, France

Molecular Detection of Bartonella quintana, B. koehlerae, B. henselae, B. clarridgeiae, Rickettsia felis, and Wolbachia pipientis in Cat Fleas, France

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