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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Salim, Shaik Mohamed Agarwal, Ajay Widjaja, Effendi Garland, Marc V. Fang, Cheng Kwong, Dim-Lee Buddharaju, Kavitha Devi Khalid, Nizamudin Mohamed Balasubramanian, Narayanan |
| Description | Country affiliation: Singapore Author Affiliation: Fang C ( Institute of Microelectronics, A STAR (Agency for Science, Technology and Research), Singapore Science Park II, Singapore. fangc@ime.a-star.edu.sg) |
| Abstract | A technique is demonstrated to detect DNA hybridization at low concentrations, based on Surface-Enhanced Raman Scattering (SERS) using silicon nanostructures coated with gold-silver as substrate. Standard silicon process technologies were employed to fabricate the SERS substrates featuring nanogaps with a characteristic distance of 15+/-10nm. Target DNA was hybridized with cysteine-modified Peptide Nucleic Acids (PNA), which was previously fixed into the nanogaps as the capture sites. After hybridization, the introduced phosphate groups from the backbone of the target DNA showed strong affinity to an inorganic linker, Zr(4+), so that resulting in the assembly substrate-PNA-DNA-Zr. Since PNA does not possess phosphate groups, the linker is avoided when there is no hybridization from the complimentary DNA. Subsequently, the assembly of substrate-PNA-DNA-Zr was incubated with a Raman label, Rhodamine B (RB). The carboxylic acid group in RB reacted with the linker Zr(4+) allowing this Raman Label to be attached to the assembly substrate-PNA-DNA-Zr. The Raman peaks corresponding to RB were selected to detect the target DNA, with a detection limit of 1 x 10(-12)M. |
| ISSN | 09565663 |
| Issue Number | 2 |
| Volume Number | 24 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-10-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Dna Analysis Chemistry Nanostructures Nanotechnology Rhodamines Spectrum Analysis, Raman Genetics Equipment Design Equipment Failure Analysis Ultrastructure Reproducibility Of Results Sensitivity And Specificity Staining And Labeling Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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