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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Luo, Kai Sheng, Qinglin Zhang, Hongfang Zheng, Jianbin |
| Description | Country affiliation: China Author Affiliation: Sheng Q ( Institute of Analytical Science/Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi 710069, China.) |
| Abstract | The formation of neodymium hexacyanoferrate (NdHCF) nanoparticles (NPs) on the surface of glucose oxidase/chitosan (GOx/CHIT) modified glass carbon electrode induced by enzymatic reaction was described and characterized. CHIT can be used not only as enzyme immobilizer, but also to provide active sites for NPs growth. Results showed that the optimized conditions of the GOx/CHIT film induced NdHCF NPs for the biosensing of glucose were 1.0mM Nd(3+) and 20.0mM Fe(CN)(6)(3-). The biocatalyzed generation of NdHCF NPs enabled the development of an electrochemical biosensor for glucose. The calculated apparent Michaelis-Menten constant was 7.5mM. The linear range for glucose detection was 0.01-10.0mM with the correlation coefficient of 0.9946, and the detection limit was 5 microM (S/N=3). Furthermore, this system avoids the interferences of other species during the biosensing process and can be used for the determination of glucose in human plasma samples. |
| ISSN | 09565663 |
| Issue Number | 3 |
| Volume Number | 24 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-11-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Chitosan Chemistry Ferrocyanides Glucose Oxidase Glucose Analysis Metal Nanoparticles Neodymium Electrodes Microscopy, Electron, Scanning Potassium Reproducibility Of Results Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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