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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sapsford, Kim E. Kostov, Yordan Francis, Jesse Sharma, Shashi Sun, Steven Rasooly, Avraham |
| Description | Country affiliation: United States Author Affiliation: Sapsford KE ( Division of Biology, Office of Science and Engineering Laboratories, FDA, Silver Spring, MD 20993, USA.) |
| Abstract | Current biodetection illumination technologies (laser, LED, tungsten lamp, etc.) are based on spot illumination with additional optics required when spatial excitation is required. Herein we describe a new approach of spatial illumination based on electroluminescence (EL) semiconductor strips available in several wavelengths, greatly simplifying the biosensor design by eliminating the need for additional optics. This work combines EL excitation with charge-coupled device (CCD) based detection (EL-CCD detector) of fluorescence for developing a simple portable detector for botulinum neurotoxin A (BoTN-A) activity analysis. A Förster Resonance Energy Transfer (FRET) activity assay for BoTN-A was used to both characterize and optimize the EL-CCD detector. The system consists of two modules: (1) the detection module which houses the CCD camera and emission filters, and (2) the excitation and sample module, containing the EL strip, the excitation filter and the 9-well sample chip. The FRET activity assay used in this study utilized a FITC/DABCYL-SNAP-25 peptide substrate in which cleavage of the substrate by BoTN-A, or its light chain derivative (LcA), produced an increase in fluorescence emission. EL-CCD detector measured limits of detection (LODs) were similar to those measured using a standard fluorescent plate reader with valves between 0.625 and 1.25 nM (31-62 ng/ml) for LcA and 0.313 nM (45 ng/ml) for the full toxin, BoTN-A. As far as the authors are aware this is the first demonstration of phosphor-based EL strips being used for the spatial illumination/excitation of a surface, coupled with CCD for point of care detection. |
| ISSN | 09565663 |
| e-ISSN | 18734235 |
| DOI | 10.1016/j.bios.2008.06.018 |
| Journal | Biosensors and Bioelectronics |
| Issue Number | 4 |
| Volume Number | 24 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-12-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Biosensing Techniques Instrumentation Botulinum Toxins, Type A Electrochemistry Luminescent Measurements Point-of-care Systems Spectrometry, Fluorescence Equipment Design Equipment Failure Analysis Reproducibility Of Results Sensitivity And Specificity Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s. Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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