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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ganjali, Mohammad Reza Rafiee-Pour, Hossain-Ali Ghourchian, Hedayatollah Rahimi, Parvaneh Norouzi, Parviz |
| Description | Country affiliation: Iran Author Affiliation: Rahimi P ( Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran.) |
| Abstract | A nano-composite material consisting of amine functionalized multi-walled carbon nanotubes and a room temperature ionic-liquid (1-butyl-3-methylimidazolium tetrafluoroborate) was prepared and used to construct a novel catalase (Ct) based biosensor for the determination of hydrogen peroxide. The modified electrode exhibited a quasi-reversible cyclic voltammogram corresponding to the Fe(II)/Fe(III) redox couple in the heme prosthetic group of Ct with a formal potential of -460 mV in 0.1M phosphate buffer solution at pH 7.0. The nano-composite film showed an obvious promotion of the direct electron transfer between Ct and the underlying electrode. The apparent charge transfer rate constant and transfer coefficient for electron transfer between the electrode surface and enzyme were calculated as 2.23s(-1) and 0.45, respectively. The immobilized Ct exhibited a relatively high sensitivity (4.9 nA/nM) toward hydrogen peroxide. Under the optimized experimental conditions, hydrogen peroxide was detected in the concentration range from 8.6 to 140 nM with a detection limit of 3.7 nM at S/N=3. The modified electrode was stable for two weeks with no observable change in the cyclic voltammograms. |
| ISSN | 09565663 |
| Issue Number | 6 |
| Volume Number | 25 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-02-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Catalase Chemistry Electrochemistry Hydrogen Peroxide Ionic Liquids Microelectrodes Nanotubes, Carbon Amines Analysis Electron Transport Enzymes, Immobilized Equipment Design Equipment Failure Analysis Ultrastructure Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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