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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ma, Zhanfang Shi, Wentao |
| Description | Country affiliation: China Author Affiliation: Shi W ( Department of Chemistry, Capital Normal University, No. 105 Xi San Huan North Rd., Beijing 100048, China.) |
| Abstract | We report on the utilization of triangular silver nanoprisms (AgTNPs) to create a highly responsive glucose biosensor. Glucose oxidase (GOD) was simply mixed with AgTNPs and cross-linked on the Pt electrode with chitosan (CHIT) medium by glutaraldehyde. Circular dichroism (CD) and UV-spectrum results show that the activity of GOD was preserved after conjugating with AgTNPs. The current response of modified electrode is 16 times higher than that without AgTNPs. The biosensor showed high sensitivity (67.17 µAcm(-2)mM(-1)), low detection limit (1 × 10(-6)M), good storage stability, and high affinity to glucose (K(M)=3.84 mM). A linear calibration plot was obtained in the wide concentration range from 3 × 10(-6) to 3 × 10(-3)M. The excellent performance of the biosensor is attributed to large surface-to-volume ratio and high conductivity of AgTNPs, and good biocompatibility of CHIT, which enhances the enzyme absorption and promotes electron transfer between redox enzymes and the surface of electrodes. |
| ISSN | 09565663 |
| Issue Number | 3 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-11-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Glucose Analysis Methods Statistics & Numerical Data Chitosan Chemistry Electrochemical Techniques Enzyme Stability Enzymes, Immobilized Glucose Oxidase Metal Nanoparticles Ultrastructure Microscopy, Electron, Transmission Nanocomposites Nanotechnology Sensitivity And Specificity Silver Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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