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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kim, Byoung Chan Kim, Jungbae Ha, Su Lee, Inseon Kim, Hyeongseok Lee, Jung-heon Kwon, Yongchai |
| Description | Author Affiliation: Kim H ( Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.); Lee I ( Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.); Kwon Y ( Graduate School of Energy and Environment, Seoul National University of Science and Technology, Seoul 139-743, Republic of Korea.); Kim BC ( Environment Division, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.); Ha S ( School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-2710, USA.); Lee JH ( Department of Chemical Engineering, Chosun University, Gwangju 501-759, Republic of Korea.); Kim J ( Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.) |
| Abstract | Glucose oxidase (GOx) was immobilized into the porous matrix of polyaniline nanofibers in a three-step process, consisting of enzyme adsorption, precipitation, and crosslinking (EAPC). EAPC was highly active and stable when compared to the control samples of enzyme adsorption (EA) and enzyme adsorption and crosslinking (EAC) with no step of enzyme precipitation. The GOx activity of EAPC was 9.6 and 4.2 times higher than those of EA and EAC, respectively. Under rigorous shaking at room temperature for 56 days, the relative activities of EA, EAC and EAPC, defined as the percentage of residual activity to the initial activity, were 22%, 19% and 91%, respectively. When incubated at 50°C under shaking for 4h, EAPC showed a negligible decrease of GOx activity while the relative activities of EA and EAC were 45% and 48%, respectively. To demonstrate the feasible application of EAPC in biofuel cells, the enzyme anodes were prepared and used for home-built air-breathing biofuel cells. The maximum power densities of biofuel cells with EA and EAPC anodes were 57 and 292 µW/cm(2), respectively. After thermal treatment at 60°C for 4h, the maximum power density of EA and EAPC anodes were 32 and 315 µW/cm(2), representing 56% and 108% of initially obtained maximum power densities, respectively. Because the lower power densities and short lifetime of biofuel cells are serious problems against their practical applications, the present results with EAPC anode has opened up a new potential for the realization of practical biofuel cell applications. |
| ISSN | 09565663 |
| Issue Number | 9 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bioelectric Energy Sources Biosensing Techniques Glucose Oxidase Chemistry Nanofibers Aniline Compounds Enzymes, Immobilized Glucose Laccase Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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