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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rosser, Charles J. Prabhulkar, Shradha Zhu, Xuena Almeide, Eugenio Vandenberg, Katherine Methee, Kalai Schneper, Lisa Li, Chen-zhong |
| Description | Country affiliation: United States Author Affiliation: Li CZ ( Nanobioengineering/Bioelectronics Lab, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA. licz@fiu.edu) |
| Abstract | Point-of-care testing (POCT) of infectious bacterial agents offers substantial benefits for disease diagnosis, mainly by shortening the time required to obtain results and by making the test available bedside or at remote care centers. Immunochromatographic lateral flow biosensors offer a low cost, highly sensitive platform for POCT. In this article, we describe the fabrication and testing of a multiplex immuno-disc sensor for the specific detection of Pseudomonas aeruginosa and Staphylococcus aureus. Antibody conjugated gold nanoparticles were used as the signaling agents. The detection range of the bacteria lies within 500-5000 CFU/ml. The advantage of the immuno-disc sensor is that it does not require any preprocessing of biological sample and is capable of whole cell bacterial detection. We also describe the design and fabrication of a compact portable device which converts the color intensity of the gold nanoparticles that accumulate at the test region into a quantitative voltage reading proportional to the bacterial concentration in the sample. The combination of the immuno-disc and the portable color reader provides a rapid, sensitive, low cost, and quantitative tool for the detection of a panel of infectious agents present in the patient sample. |
| ISSN | 09565663 |
| Issue Number | 11 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-07-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacteriological Techniques Methods Biosensing Techniques Point-of-care Systems Antibodies, Bacterial Antibodies, Immobilized Bacterial Infections Diagnosis Instrumentation Equipment Design Gold Humans Metal Nanoparticles Paper Pseudomonas Aeruginosa Immunology Isolation & Purification Staphylococcus Aureus Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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