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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lei, Kin Fong Chen, Yi-Dao Hsu, Che-Wei Wu, Min-Hsien |
| Description | Author Affiliation: Lei KF ( Graduate Institute of Medical Mechatronics, Chang Gung University, Taoyuan, Taiwan ROC) |
| Abstract | A perfusion three-dimensional (3D) cell culture microfluidic chip has been developed for real-time and non-invasive impedimetric monitoring of cell proliferation and chemosensitivity. In this study, human oral cancer cells (OEC-M1) were encapsulated in 3D agarose scaffold and cultured in a miniaturized chamber under perfusion of tested substance. This setting provides a more in vitro physiologically relevant microenvironment to better mimic the complex in vivo microenvironment. A pair of vertical electrodes was embedded at the opposite sidewalls of the culture chamber for the on-site impedance measurement. Cell density in the 3D construct was shown to be proportional to the impedance magnitude of the entire construct. Therefore, perfusion 3D cell culture was performed for up to 5 days and cell proliferation can be monitored by the impedimetric analysis. Moreover, real-time impedimetric monitoring of cell viability under the perfusion of anti-cancer drug in different concentrations was conducted and the impedance magnitude was directly correlated with the cell viability. From the confirmation of the endpoint cell viability assays, a concentration-dependent effect was shown; however, the response of cell viability during the drug treatment was able to be traced by the impedance measurement. The experimental results showed that cell proliferation and chemosensitivity in 3D cell culture format can be monitored by impedance measurement. This microfluidic chip has a high potential to develop a powerful analytical platform for cancer research. |
| ISSN | 09565663 |
| Volume Number | 51 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cell Culture Techniques Instrumentation Drug Screening Assays, Antitumor Microfluidic Analytical Techniques Antineoplastic Agents Pharmacology Biosensing Techniques Cell Line, Tumor Cell Proliferation Drug Effects Cell Survival Electric Impedance Equipment Design Humans Mouth Neoplasms Drug Therapy Sepharose Chemistry Tissue Scaffolds Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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