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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Xiros, Charilaos Katapodis, Petros Topakas, Evangelos Christakopoulos, Paul |
| Description | Country affiliation: Greece Author Affiliation: Xiros C ( Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Strasse, Zografou Campus, 15700 Athens, Greece.) |
| Abstract | In this study, the ethanol production by the mesophilic fungus Neurospora crassa from BG was studied and optimized concerning the induction of lignocellulose degrading enzymes and the production phase as well. The production of cellulolytic and hemicellulolytic enzymes was studied under solid-state cultivation (SSC). SSC in a laboratory horizontal bioreactor using the optimized medium, WS and BG in the ratio 1:1 and initial moisture level 61.5%, allowed the large scale production of the multienzymatic system. Similar yields with those from flasks experiments, as high as 1073,56,4.2,1.6,3.1,5.7 and 0.52 U g(-1) carbon source of xylanase, endoglucanase, cellobiohydrolase, beta-glucosidase, alpha-l-arabinofuranosidase, acetyl esterase and feruloyl esterase, respectively, were obtained. Chromogenic (fluorogenic) 4-methylumbelliferyl substrates were used to characterize the major activities of the multienzyme component, after the separation by isoelectric focusing (IEF) electrophoresis. Alkali pre-treated BG was used for ethanol production. A yield of about 74 g of ethanol kg(-1) dry BG (5,6 g L(-1)) was obtained under optimum conditions (aeration 0.1 vvm, pre-treatment with 1g NaOH 10 g(-1)dry BG). |
| ISSN | 09608524 |
| Issue Number | 13 |
| Volume Number | 99 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-09-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Ethanol Metabolism Fermentation Hydrolases Neurospora Crassa Enzymology Saccharomyces Cerevisiae Biomass Bioreactors Furans Hydrolysis Monosaccharides Substrate Specificity Sulfuric Acids Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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