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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Banik, R. M. Pandey, S. K. |
| Description | Country affiliation: India Author Affiliation: Pandey SK ( School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.) |
| Abstract | A study was made to find out maximum partitioning of Bacillus licheniformis alkaline phosphatase in different ATPSs composed of different molecular weight of PEG X (X=2000, 4000, 6000) with salts (magnesium sulphate, sodium sulphate, sodium citrate) and polymers (dextran 40, dextran T500). Physicochemical factors such as effect of system pH, system temperature and production media were evaluated for partitioning of alkaline phosphatase. PEG 4000 [9.0% (w/v)] and dextran T500 [9.6% (w/v)] were selected as most suitable system components for alkaline phosphatase production by B. licheniformis based on greater partition coefficient (k=5.23). The two-phase system produced fewer enzymes than the homogeneous fermentation (control) in early stage of fermentation, but after 72 h the enzyme produced in the control system was less than that in the ATPS. Total alkaline phosphatase yield in ATPS fermentation was 3907.01 U/ml and in homogeneous fermentation 2856.50 U/ml. |
| ISSN | 09608524 |
| Issue Number | 5 |
| Volume Number | 102 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-03-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Alkaline Phosphatase Biosynthesis Bacillus Enzymology Biotechnology Methods Dextrans Metabolism Fermentation Physiology Polyethylene Glycols Citrates Hydrogen-ion Concentration Magnesium Sulfate Sulfates Temperature Comparative Study Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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