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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ugwu, Charles U. Tokiwa, Yutaka Ichiba, Toshio |
| Description | Country affiliation: Japan Author Affiliation: Ugwu CU ( Okinawa Industrial Technology Center, Suzaki 12-2, Uruma, Okinawa 904-2234, Japan. ugutyaru@pref.okinawa.lg.jp) |
| Abstract | Feasibility of producing (R)-3-hydroxybutyric acid ((R)-3-HB) using wild type Azohydromonas lata and its mutants (derived by UV mutation) was investigated. A. lata mutant (M5) produced 780 m g/l in the culture broth when sucrose was used as the carbon source. M5 was further studied in terms of its specificity with various bioconversion substrates for production of (R)-3-HB. (R)-3-HB concentration produced in the culture broth by M5 mutant was 2.7-fold higher than that of the wild type strain when sucrose (3% w/v) and (R,S)-1,3-butanediol (3% v/v) were used as carbon source and bioconversion substrate, respectively. Bioconversion of resting cells (M5) with glucose (1% v/w), ethylacetoacetate (2% v/v), and (R,S)-1,3-butanediol (3% v/v), resulted in (R)-3-HB concentrations of 6.5 g/l, 7.3g/l and 8.7 g/l, respectively. |
| ISSN | 09608524 |
| Issue Number | 12 |
| Volume Number | 102 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-06-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | 3-hydroxybutyric Acid Biosynthesis Alcaligenaceae Metabolism Biotechnology Methods Genetics Butylene Glycols Culture Media Fermentation Mutagenesis Radiation Effects Mutation Species Specificity Sucrose Ultraviolet Rays Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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